Effect of Amalaki rasayana on DNA damage and repair in randomized aged human individuals
| Autor: | Manjunath B. Joshi, Kanive Parashiva Guruprasad, Udupi Vishwanatha, Rajeshwari Ganesh, Panniampilly M. Varier, Jayakrishna Nayak, Kollath Muraleedharan, Kapaettu Satyamoorthy, Puspendu Paladhi, Rashmi Shree, Suchitra Anchan, Bokkasa V. Prasanna, Kothanahalli S. Raghu, Raviraj Acharya, Jayalaxmi Rao, P. M. Gopinath, Thrikovil S. Muraleedharan |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Aging Time Factors DNA Repair Ultraviolet Rays DNA damage DNA repair India Fluorometric Analysis Pharmacology DNA Strand Break Young Adult 03 medical and health sciences 0302 clinical medicine Double-Blind Method Drug Discovery Humans DNA unwinding Gap filling Plant Extracts Chemistry Age Factors Base excision repair Middle Aged 030104 developmental biology Biochemistry 030220 oncology & carcinogenesis Leukocytes Mononuclear Female DNA Damage Nucleotide excision repair |
| Zdroj: | Journal of Ethnopharmacology. 191:387-397 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2016.06.062 |
| Popis: | Ethnopharmacological relevance Preparations from Phyllanthus emblica called Amalaki rasayana is used in the Indian traditional medicinal system of Ayurveda for healthy living in elderly. The biological effects and its mechanisms are not fully understood. Since the diminishing DNA repair is the hallmark of ageing, we tested the influence of Amalaki rasayana on recognized DNA repair activities in healthy aged individuals. Methods Amalaki rasayana was prepared fresh and healthy aged randomized human volunteers were administrated with either rasayana or placebo for 45 days strictly as per the traditional text. The DNA repair was analyzed in peripheral blood mononuclear cells before and after rasayana administration and after 45 days post-rasayana treatment regimen. UVC-induced DNA strand break repair (DSBR) based on extent of DNA unwinding by fluorometric analysis, nucleotide excision repair (NER) by flow cytometry and constitutive base excision repair (BER) by gap filling method were analyzed. Results Amalaki rasayana administration stably maintained/enhanced the DSBR in aged individuals. There were no adverse side effects. Further, subjects with different body mass index showed differential DNA strand break repair capacity. No change in unscheduled DNA synthesis during NER and BER was observed between the groups. Conclusion Intake of Amalaki rasayana by aged individuals showed stable maintenance of DNA strand break repair without toxic effects. However, there was no change in nucleotide and base excision repair activities. Results warrant further studies on the effects of Amalaki rasayana on DSBR activities. |
| Databáze: | OpenAIRE |
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