Long-term recovery after bone marrow stromal cell treatment of traumatic brain injury in rats
| Autor: | Michael Chopp, Changsheng Qu, Dunyue Lu, Anton Goussev, Asim Mahmood |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Traumatic brain injury medicine.medical_treatment Neurotrophic factors medicine Animals Rats Wistar Saline Bone Marrow Transplantation Cell Proliferation business.industry Cell growth Growth factor medicine.disease Immunohistochemistry Rats Treatment Outcome Nerve growth factor medicine.anatomical_structure Brain Injuries Immunology Female Bone marrow Stromal Cells business |
| Zdroj: | Journal of Neurosurgery. 104:272-277 |
| ISSN: | 0022-3085 |
| DOI: | 10.3171/jns.2006.104.2.272 |
| Popis: | Object This study was designed to follow the effects of bone marrow stromal cell (BMSC) administration in rats after traumatic brain injury (TBI) for a 3-month period. Methods Forty adult female Wistar rats were injured by a controlled cortical impact and, 1 week later, were injected intravenously with one of three different doses of BMSCs (2 × 106, 4 × 106, or 8 × 106 cells per animal) obtained in male rats. Control rats received phosphate-buffered saline (PBS). Neurological function in these rats was studied using a neurological severity scale (NSS). The rats were killed 3 months after injury, and immunohistochemical stains were applied to brain samples to study the distribution of the BMSCs. Additional brain samples were analyzed by quantitative enzyme-linked immunosorbent assays to measure the expression of the growth factors brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Three months after injury, BMSCs were present in the injured brain and their number was significantly greater in animals that received 4 × 106 or 8 × 106 BMSCs than in animals that received 2 × 106 BMSCs. The cells were primarily distributed around the lesion boundary zone. Functional outcome was significantly better in rats that received 4 × 106 or 8 × 106 BMSCs, compared with control animals, although no improvement was seen in animals that received 2 × 106 BMSCs. All doses of BMSCs significantly increased the expression of BDNF but not that of NGF; however, this increase was significantly larger in animals that received 4 × 106 or 8 × 106 BMSCs than in controls or animals that received 2 × 106 BMSCs. Conclusions In summary, when injected in rats after TBI, BMSCs are present in the brain 3 months later and significantly improve functional outcome. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|