Horizontal transfer of class 1 integrons from uropathogenic Escherichia coli to E. coli K12
| Autor: | Magela Laviña, María Eloisa Poey |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gene Transfer Horizontal 030106 microbiology Microbial Sensitivity Tests medicine.disease_cause Integron Microbiology Integrons 03 medical and health sciences Plasmid Bacterial Proteins Transduction Genetic Drug Resistance Bacterial medicine Humans Uropathogenic Escherichia coli Replicon Escherichia coli Genetics Dihydropteroate Synthase Escherichia coli K12 biology Escherichia coli Proteins biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Anti-Bacterial Agents Multiple drug resistance 030104 developmental biology Infectious Diseases Genes Bacterial Conjugation Genetic Horizontal gene transfer biology.protein bacteria Mobile genetic elements Carrier Proteins Bacteria |
| Zdroj: | Microbial Pathogenesis. 117:16-22 |
| ISSN: | 0882-4010 |
| Popis: | Class 1 integrons are genetic elements that carry a variable set of antibiotic resistance genes, being frequently found in clinical Gram-negative isolates. It is generally assumed that they easily spread horizontally among bacteria, thus contributing to the appearance of multidrug resistant clones. However, there are few experimental studies on the lateral transfer of these elements performed with bacterial collections that had been gathered following an epidemiological design. In this work, a collection with these characteristics, comprising uropathogenic Escherichia coli (UPEC) isolates bearing class 1 integrons, was employed to study the horizontal transfer of the integron to an E. coli K12 strain by means of conjugation and transduction experiments. Donor and resultant strains were characterized for their antibiotic resistances, presence of sul1, sul2 and sul3 genes, integron cassette arrays, plasmid replicons and tra region. Conjugation assays were carried out using 45 UPEC isolates as integron donors and transconjugants were obtained in 18 cases (40%). P1-transduction experiments only added the integron transfer from a single donor isolate. Thus, a collection of E. coli K12 strains carrying the class 1 integron from 19 UPEC isolates was generated. In all cases, the integron was co-transferred with at least one low-copy-number plasmid, generally of the F replicon type. Several variables were searched for that could be related to the ability to horizontally transfer the integron. Although no strict correlation was observed, the phylogenetic background of the donor strain and the presence of the sul2 gene appeared as candidates to influence the process. Therefore, there appears that besides being carried by mobile genetic elements, class 1 integrons may be influenced by other factors to accomplish their horizontal transfer, a topic that requires further studies. |
| Databáze: | OpenAIRE |
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