Ostreid herpesvirus in wild oysters from the Huelva coast (SW Spain)
| Autor: | J R López-Fernández, J.I. Navas, R De la Herrán, M. López-Sanmartín, M E Cunha |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oyster Mitochondrial DNA Population Zoology Aquatic Science law.invention 03 medical and health sciences Species Specificity law biology.animal Animals Crassostrea education Ostrea stentina Herpesviridae Ecology Evolution Behavior and Systematics Polymerase chain reaction education.field_of_study biology 04 agricultural and veterinary sciences biology.organism_classification Fishery Restriction enzyme 030104 developmental biology Haplotypes Spain Host-Pathogen Interactions 040102 fisheries 0401 agriculture forestry and fisheries Restriction fragment length polymorphism |
| Zdroj: | Diseases of Aquatic Organisms. 120:231-240 |
| ISSN: | 1616-1580 0177-5103 |
| Popis: | This is the first report of ostreid herpesvirus 1 microvariant (OsHV-1 µVar) infecting natural oyster beds located in Huelva (SW Spain). The virus was detected in 3 oyster species present in the intertidal zone: Crassostrea gigas (Thunberg, 1793), C. angulata (Lamarck, 1819) and, for the first time, in Ostrea stentina Payraudeau, 1826. Oysters were identified by a specific polymerase chain reaction (PCR) and posterior restriction fragment length polymorphism (RFLP) analysis based on cytochrome oxidase I (COI) mitochondrial DNA. Results confirmed that C. angulata still remains the dominant oyster population in SW Spain despite the introduction of C. gigas for cultivation in the late 1970s, and its subsequent naturalization. C. angulata shows a higher haplotype diversity than C. gigas. OsHV-1 virus was detected by PCR with C2/C6 pair primers. Posterior RFLP analyses with the restriction enzyme MfeI were done in order to reveal the OsHV-1 µVar. Detections were confirmed by DNA sequencing, and infections were evidenced by in situ hybridization in C. gigas, C. angulata and O. stentina samples. The prevalence was similar among the 3 oyster species but varied between sampling locations, being higher in areas with greater harvesting activities. OsHV-1 µVar accounted for 93% of all OsHV-1 detected. |
| Databáze: | OpenAIRE |
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