The superiority of conditioned medium derived from rapidly expanded mesenchymal stem cells for neural repair

Autor: Ya Tzu Chen, Henrich Cheng, Ming Jei Lo, Meng-Jen Lee, Nini Hsieh, Wen Cheng Huang, May Jywan Tsai
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Neurite
Cell Culture Techniques
Medicine (miscellaneous)
Spinal cord injury
Mesenchymal Stem Cell Transplantation
Biochemistry
Genetics and Molecular Biology (miscellaneous)
Regenerative medicine
Cell Line
lcsh:Biochemistry
Secreted factors
Rats
Sprague-Dawley
03 medical and health sciences
Random Allocation
0302 clinical medicine
In vivo
medicine
Animals
Humans
CD90
lcsh:QD415-436
Spinal Cord Injuries
Mesenchymal stem cell
lcsh:R5-920
Xeno-free medium
Epidermal Growth Factor
Chemistry
Research
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
Cell biology
Nerve Regeneration
Rats
030104 developmental biology
medicine.anatomical_structure
Culture Media
Conditioned
Molecular Medicine
Female
Fibroblast Growth Factor 2
Bone marrow
Neuron
Microglia
Stem cell
lcsh:Medicine (General)
030217 neurology & neurosurgery
Zdroj: Stem Cell Research & Therapy
Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-15 (2019)
ISSN: 1757-6512
Popis: s Background Spinal cord injury (SCI) is a complex and severe neurological condition. Mesenchymal stem cells (MSCs) and their secreted factors show promising potential for regenerative medicine. Many studies have investigated MSC expansion efficacy of all kinds of culture medium formulations, such as growth factor-supplemented or xeno-free medium. However, very few studies have focused on the potential of human MSC (hMSC) culture medium formulations for injured spinal cord repair. In this study, we investigated the effect of hMSC-conditioned medium supplemented with bFGF, EGF, and patient plasma, namely, neural regeneration laboratory medium (NRLM), on SCI in vitro and in vivo. Methods Commercial and patient bone marrow hMSCs were obtained for cultivation in standard medium and NRLM separately. Several characteristics, including CD marker expression, differentiation, and growth curves, were compared between MSCs cultured in standard medium and NRLM. Additionally, we investigated the effect of the conditioned medium (referred to as NRLM-CM) on neural repair, including inflammation inhibition, neurite regeneration, and spinal cord injury (SCI), and used a coculture system to detect the neural repair function of NRLM-MSCs. Results Compared to standard culture medium, NRLM-CM had superior in inflammation reduction and neurite regeneration effects in vitro and improved functional restoration in SCI rats in vivo. In comparison with standard culture medium MSCs, NRLM-MSCs proliferated faster regardless of the age of the donor. NRLM-MSCs also showed increased adipose differentiative potential and reduced CD90 expression. Both types of hMSC CM effectively enhanced injured neurite outgrowth and protected against H2O2 toxicity in spinal cord neuron cultures. Cytokine arrays performed in hMSC-CM further revealed the presence of at least 120 proteins. Among these proteins, 6 demonstrated significantly increased expression in NRLM-CM: adiponectin (Acrp30), angiogenin (ANG), HGF, NAP-2, uPAR, and IGFBP2. Conclusions The NRLM culture system provides rapid expansion effects and functional hMSCs. The superiority of the derived conditioned medium on neural repair shows potential for future clinical applications.
Databáze: OpenAIRE
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