The superiority of conditioned medium derived from rapidly expanded mesenchymal stem cells for neural repair
Autor: | Ya Tzu Chen, Henrich Cheng, Ming Jei Lo, Meng-Jen Lee, Nini Hsieh, Wen Cheng Huang, May Jywan Tsai |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Neurite Cell Culture Techniques Medicine (miscellaneous) Spinal cord injury Mesenchymal Stem Cell Transplantation Biochemistry Genetics and Molecular Biology (miscellaneous) Regenerative medicine Cell Line lcsh:Biochemistry Secreted factors Rats Sprague-Dawley 03 medical and health sciences Random Allocation 0302 clinical medicine In vivo medicine Animals Humans CD90 lcsh:QD415-436 Spinal Cord Injuries Mesenchymal stem cell lcsh:R5-920 Xeno-free medium Epidermal Growth Factor Chemistry Research Cell Differentiation Mesenchymal Stem Cells Cell Biology Cell biology Nerve Regeneration Rats 030104 developmental biology medicine.anatomical_structure Culture Media Conditioned Molecular Medicine Female Fibroblast Growth Factor 2 Bone marrow Neuron Microglia Stem cell lcsh:Medicine (General) 030217 neurology & neurosurgery |
Zdroj: | Stem Cell Research & Therapy Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-15 (2019) |
ISSN: | 1757-6512 |
Popis: | s Background Spinal cord injury (SCI) is a complex and severe neurological condition. Mesenchymal stem cells (MSCs) and their secreted factors show promising potential for regenerative medicine. Many studies have investigated MSC expansion efficacy of all kinds of culture medium formulations, such as growth factor-supplemented or xeno-free medium. However, very few studies have focused on the potential of human MSC (hMSC) culture medium formulations for injured spinal cord repair. In this study, we investigated the effect of hMSC-conditioned medium supplemented with bFGF, EGF, and patient plasma, namely, neural regeneration laboratory medium (NRLM), on SCI in vitro and in vivo. Methods Commercial and patient bone marrow hMSCs were obtained for cultivation in standard medium and NRLM separately. Several characteristics, including CD marker expression, differentiation, and growth curves, were compared between MSCs cultured in standard medium and NRLM. Additionally, we investigated the effect of the conditioned medium (referred to as NRLM-CM) on neural repair, including inflammation inhibition, neurite regeneration, and spinal cord injury (SCI), and used a coculture system to detect the neural repair function of NRLM-MSCs. Results Compared to standard culture medium, NRLM-CM had superior in inflammation reduction and neurite regeneration effects in vitro and improved functional restoration in SCI rats in vivo. In comparison with standard culture medium MSCs, NRLM-MSCs proliferated faster regardless of the age of the donor. NRLM-MSCs also showed increased adipose differentiative potential and reduced CD90 expression. Both types of hMSC CM effectively enhanced injured neurite outgrowth and protected against H2O2 toxicity in spinal cord neuron cultures. Cytokine arrays performed in hMSC-CM further revealed the presence of at least 120 proteins. Among these proteins, 6 demonstrated significantly increased expression in NRLM-CM: adiponectin (Acrp30), angiogenin (ANG), HGF, NAP-2, uPAR, and IGFBP2. Conclusions The NRLM culture system provides rapid expansion effects and functional hMSCs. The superiority of the derived conditioned medium on neural repair shows potential for future clinical applications. |
Databáze: | OpenAIRE |
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