3D bioprinting of integral ADSCs-NO hydrogel scaffolds to promote severe burn wound healing
| Autor: | Guo Wang, Yuansen Luo, Shihai Jiang, Tangzhao Liang, Chang Liu, Ying Hu, Lei Zhu, Tao Xu, Jing Zhang, Yu Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Angiogenesis
wound healing 02 engineering and technology severe burn law.invention Biomaterials Neovascularization 03 medical and health sciences chemistry.chemical_compound angiogenesis law nitric oxide ADSCs medicine 030304 developmental biology 0303 health sciences 3D bioprinting business.industry Mesenchymal stem cell 021001 nanoscience & nanotechnology Vascular endothelial growth factor Vascular endothelial growth factor A chemistry Cancer research AcademicSubjects/SCI01410 Stem cell medicine.symptom 0210 nano-technology Wound healing business AcademicSubjects/MED00010 Research Article |
| Zdroj: | Regenerative Biomaterials |
| ISSN: | 2056-3426 2056-3418 |
| Popis: | Severe burns are challenging to heal and result in significant death throughout the world. Adipose-derived mesenchymal stem cells (ADSCs) have emerged as a promising treatment for full-thickness burn healing but are impeded by their low viability and efficiency after grafting in vivo. Nitric oxide (NO) is beneficial in promoting stem cell bioactivity, but whether it can function effectively in vivo is still largely unknown. In this study, we bioprinted an efficient biological scaffold loaded with ADSCs and NO (3D-ADSCs/NO) to evaluate its biological efficacy in promoting severe burn wound healing. The integral 3D-ADSCs/NO hydrogel scaffolds were constructed via 3D bioprinting. Our results shown that 3D-ADSCs/NO can enhance the migration and angiogenesis of Human Umbilical Vein Endothelial Cells (HUVECs). Burn wound healing experiments in mice revealed that 3D-ADSCs/NO accelerated the wound healing by promoting faster epithelialization and collagen deposition. Notably, immunohistochemistry of CD31 suggested an increase in neovascularization, supported by the upregulation of vascular endothelial growth factor (VEGF) mRNA in ADSCs in the 3D biosystem. These findings indicated that 3D-ADSC/NO hydrogel scaffold can promote severe burn wound healing through increased neovascularization via the VEGF signalling pathway. This scaffold may be considered a promising strategy for healing severe burns. |
| Databáze: | OpenAIRE |
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