Liquid holding increases mutation induction by formaldehyde and some other cross-linking agents in Escherichia coli K12
| Autor: | J.A. Zijlstra |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Tris
Time Factors Health Toxicology and Mutagenesis Drug Resistance Formaldehyde medicine.disease_cause Nalidixic Acid chemistry.chemical_compound Escherichia coli Genetics medicine Molecular Biology Strain (chemistry) biology Mutagenicity Tests Chemistry Mutagenesis biology.organism_classification Enterobacteriaceae Cross-Linking Reagents Biochemistry Mutation Amine gas treating Bacteria DNA Damage |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 210:255-261 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/0027-5107(89)90086-9 |
| Popis: | The induction by some cross-linking agents of forward mutations leading to nalidixic acid resistance in Escherichia coli K12/343/113 was considerably enhanced when a 24-h period of liquid holding was interpolated between treatment and growth phase. Liquid holding increased the mutagenic effectiveness of nor-nitrogen mustard (NNM) 28-fold, of phosphoramide mustar (PAM) 10-fold, and of tris-ethylen-eimino)-phosphineoxide (TEPA), tris(chloroethyl)amine (TCEA) and chloracetaldehyde (CAA) 3-fold, over the complete concentration range. By contrast, the activities of cisplatin (CDDP), transplatin (TDDP) and chloracetamide-N-metholol (CAM) were slightly decreased after liquid holding. Liquid holding did not measurably influence the mutagenicity of formadehyde at low concentrations, whereas at higher concentrations an 8-fold increase was observed. As opposed to the considerable activity in the Uvr+ strain, formaldehyde was found not to be mutagenically active in an E. coli strain carrying a deletion of the uvrB gene. |
| Databáze: | OpenAIRE |
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