Mosquitocidal and Anti-Inflammatory Properties of The Essential Oils Obtained from Monoecious, Male, and Female Inflorescences of Hemp (Cannabis sativa L.) and Their Encapsulation in Nanoemulsions

Autor: Oliviero Marinelli, Irene Ricci, Giulia Bonacucina, Pierluigi Pompei, Massimo Nabissi, Filippo Maggi, Lucia Pavoni, Eugenia Mazzara, Paolo Rossi, Alessia Cappelli, Matteo Valzano, Riccardo Petrelli
Rok vydání: 2020
Předmět:
Male
0106 biological sciences
Insecticides
Anti-Inflammatory Agents
Pharmaceutical Science
01 natural sciences
Analytical Chemistry
law.invention
chemistry.chemical_compound
law
Drug Discovery
Food science
Inflorescence
anti-inflammatory
biology
Anopheles stephensi
Chemistry (miscellaneous)
Molecular Medicine
Emulsions
Female
medicine.drug_class
malaria
Cannabis sativa
Sesquiterpene
Article
Gas Chromatography-Mass Spectrometry
Anti-inflammatory
Industrial waste
lcsh:QD241-441
lcsh:Organic chemistry
Oils
Volatile
medicine
Animals
Humans
Plant reproductive morphology
Physical and Theoretical Chemistry
Essential oil
Cannabis
Anopheles gambiae
Dose-Response Relationship
Drug
010405 organic chemistry
Organic Chemistry
hemp
biology.organism_classification
0104 chemical sciences
Culicidae
chemistry
green pesticides
010606 plant biology & botany
Zdroj: Molecules
Volume 25
Issue 15
Molecules, Vol 25, Iss 3451, p 3451 (2020)
ISSN: 1420-3049
DOI: 10.3390/molecules25153451
Popis: Among the various innovative products obtainable from hemp (Cannabis sativa L.) waste biomass originating from different industrial processes, the essential oil (EO) deserves special attention in order to understand its possible application in different fields, such as cosmetics, pharmaceuticals, and botanical insecticides. For the purpose, in the present work, we studied the chemical composition of EOs obtained from different hemp varieties, namely Felina 32 and Carmagnola Selezionata (CS) using monoecious, male, and female inflorescences, and we evaluated their mosquitocidal activities on larvae and pupae of two main malaria vectors, Anopheles gambiae and An. stephensi. Then, in order to evaluate the safe use of hemp EOs for operators, the potential pro- or anti-inflammatory effect of hemp EOs together with their toxicological profile were determined on dermal fibroblasts and keratinocytes. Given the promising results obtained by insecticidal and anti-inflammatory studies, a preliminary evaluation of EOs encapsulation into nanoemulsions (NEs) has been performed with the aim to develop a formulation able to improve their poor physicochemical stability. Felina 32 and CS inflorescences provided EOs with an interesting chemical profile, with monoterpene and sesquiterpene hydrocarbons as the major components. This study highlighted the potential application of male inflorescences, which are usually discharged during hemp product processing. These EOs could be exploited as potential sustainable and eco-friendly insecticides, given their capability to be toxic against mosquitoes and the possibility to use them to prepare stable and safe formulations. The LC50 values found in this study (<
80 ppm) are lower, on average, than those of many plant EOs, with the advantage of using an industrial waste product. From MTT assay and gene and protein expression analysis, EOs showed no cytotoxicity at the appropriate doses and exerted an anti-inflammatory effect on the human cell lines tested. These findings encourage further applied research on hemp EOs in order support their industrial exploitation.
Databáze: OpenAIRE
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