Novel pectin-like polysaccharide from Panax notoginseng attenuates renal tubular cells fibrogenesis induced by TGF-β
| Autor: | Qianhu Wu, Chunfan Huang, Xiaoqi Jing, Kan Ding |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Epithelial-Mesenchymal Transition
Polymers and Plastics Panax notoginseng Vimentin Pharmacology Kidney Polysaccharide Cell Line Transforming Growth Factor beta Materials Chemistry Renal fibrosis Humans Epithelial–mesenchymal transition chemistry.chemical_classification biology Chemistry Organic Chemistry Epithelial Cells Cadherins biology.organism_classification Fibrosis Actins Fibronectins Fibronectin Kidney Tubules SNAI1 biology.protein Pectins Kidney Diseases Transforming growth factor |
| Zdroj: | Carbohydrate Polymers. 276:118772 |
| ISSN: | 0144-8617 |
| Popis: | Renal fibrosis is the final common result of a variety of progressive injuries leading to chronic renal failure. However, there are no effective clinical available drugs for the treatment. Notoginsenoside from Panax notoginseng could ameliorate renal fibrosis. We hypothesized that polysaccharide from this herb might have similar bioactivity. Here, we elucidated structure of a novel pectin-like polysaccharide designed SQD4S2 with a netty antenna backbone of glucogalacturonan substituted by glucoarabinan, glucurogalactan and galactose residues from this herb. Interestingly, SQD4S2 could reverse the morphological changes of human renal tubular HK-2 cells induced by TGF-β. Mechanism study suggested that this bioactivity might associate with N-cadherin (CDH2), Snail (SNAI1), Slug (SNAI2) depression and E-cadherin (CDH1) enhancement. In addition, SQD4S2 could impede critical fibrogenesis associated molecules such as α-SMA, fibronectin, vimentin, COL1A1, COL3A1, FN1 and ACTA2 expression induced by TGF-β in HK-2 cells. Current findings outline a novel leading polysaccharide for against renal fibrosis new drug development. |
| Databáze: | OpenAIRE |
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