Increased spontaneous release of tumour necrosis factor-alpha by alveolar macrophages from wheezy infants
| Autor: | Claude Dumarey, M. Bachelet, P. Scheinmann, Inês Azevedo, B. Boris Vargaftig, J. de Blic |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Lipopolysaccharides
Male Pulmonary and Respiratory Medicine medicine.medical_specialty Necrosis medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Dexamethasone Dinoprostone chemistry.chemical_compound Recurrence Reference Values Internal medicine Bronchoscopy Macrophages Alveolar medicine Humans Prostaglandin E2 Glucocorticoids Respiratory Sounds medicine.diagnostic_test Tumor Necrosis Factor-alpha business.industry Infant Radioimmunoassay Thromboxane B2 Endocrinology Bronchoalveolar lavage medicine.anatomical_structure Cytokine chemistry Child Preschool Immunology Female medicine.symptom Pulmonary alveolus business Bronchoalveolar Lavage Fluid medicine.drug |
| Zdroj: | European Respiratory Journal. 10:1767-1773 |
| ISSN: | 1399-3003 0903-1936 |
| Popis: | We determined if alveolar macrophages (AMs) from infants with severe recurrent wheezing episodes release increased amounts of tumour necrosis factor-alpha (TNF-alpha), as described in adults with asthma. We compared TNF-alpha release by unstimulated and lipopolysaccharide-stimulated AMs obtained by bronchoalveolar lavage in 13 wheezy and seven nonwheezy infants (aged 6-36 months) and analysed its regulation by dexamethasone. Metabolites in cell supernatants were quantified by enzyme-linked immunosorbent assay (ELISA) (TNF-alpha) or radioimmunoassay (thromboxane B2 and prostaglandin E2). Comparison of results was performed by the Mann-Whitney U-test and values were expressed as median (interquartile range) in ng x 10(6) cells(-1). Resting AMs from wheezy infants released larger amounts of TNF-alpha and thromboxane B2 as compared to controls: 2.67 (0.89-8.33) vs 0.48 (0.25-1.08) and 75.63 (38.07-158.91) vs 10.03 (7.36-76.08), respectively (p |
| Databáze: | OpenAIRE |
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