Targeting out of range biomolecules: Chemical labeling strategies for qualitative and quantitative MALDI MS-based detection
| Autor: | P. Bruyat, L. Devel, G. Subra, S. Cantel, C. Malgorn, S. Bregant, M. Sejalon-Cipolla |
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| Přispěvatelé: | Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO) (SIMoS), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), ANR-18-CE44-0012,ProMaP,Nouvelles sondes pour la détection de Métallo protéases par spectrométrie de masse(2018) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Maldi ms Chemistry Biomolecule [SDV]Life Sciences [q-bio] 010401 analytical chemistry Research context Computational biology 010402 general chemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry Signal enhancement [CHIM]Chemical Sciences Spectroscopy Chemical labeling |
| Zdroj: | Trends in Analytical Chemistry Trends in Analytical Chemistry, 2021, 143, pp.116399. ⟨10.1016/j.trac.2021.116399⟩ |
| ISSN: | 0165-9936 |
| DOI: | 10.1016/j.trac.2021.116399⟩ |
| Popis: | MALDI-MS is of crucial importance for detection and identification of relevant molecules involved in biological pathways allowing a better understanding of physiological processes advantageously related to diagnosis strategies. However, many relevant biomarkers remain undetectable due to several parameters including low molecular weight, weak abundance, MS ionization potential or localization in a complex environment. To overcome these limitations, labeling strategies were developed to target reactive organic moieties of biomolecules of interest. More recently, activity-based probes or antibodies were implemented to match the current need for in vivo biomolecule specific labeling. Furthermore, with the development of MALDI-MS imaging, matrix-free derivatization techniques are currently in the spotlight. MALDI-MS labeling requires the development of a panel of tags designed to target different functional groups promoting signal enhancement for many biomolecules. This review recapitulates recent derivatization methods and available labeling reagents while putting into perspective the importance of MALDI-MS labels in the biochemical research context. |
| Databáze: | OpenAIRE |
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