Identification of a Novel Neuromedin U Receptor Subtype Expressed in the Central Nervous System
| Autor: | James H. Crona, Thomas M. Laz, Xudong Qiao, Joseph A. Hedrick, Suke Wang, Frederick J. Monsma, LiXin Shan, Marvin L. Bayne, Eric L. Gustafson, Jiang Behan |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Central Nervous System
medicine.medical_specialty DNA Complementary Time Factors Databases Factual Molecular Sequence Data Neuropeptide Biology Ligands Biochemistry Mice Neuromedin U receptor Internal medicine Complementary DNA Calcium flux medicine Animals Humans Tissue Distribution Amino Acid Sequence RNA Messenger Cloning Molecular Receptor Molecular Biology Dose-Response Relationship Drug Sequence Homology Amino Acid Neuropeptides HEK 293 cells Membrane Proteins Cell Biology Blotting Northern Molecular biology Receptors Neurotransmitter Endocrinology Autoradiography Calcium Neuromedin S Neuromedin U |
| Zdroj: | Journal of Biological Chemistry. 275:39482-39486 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.c000522200 |
| Popis: | Neuromedin U is a neuropeptide prominently expressed in the upper gastrointestinal tract and central nervous system. Recently, GPR66/FM-3 (NmU-R1) was identified as a specific receptor for neuromedin U. A BLAST search of the GenBankTM genomic database using the NmU-R1 cDNA sequence revealed a human genomic fragment encoding a G protein-coupled receptor that we designated NmU-R2 based on its homology to NmU-R1. The full-length NmU-R2 cDNA was subsequently cloned, stably expressed in 293 cells, and shown to mobilize intracellular calcium in response to neuromedin U. This response was dose-dependent (EC50 = 5 nm) and specific in that other neuromedins did not induce a calcium flux in receptor-transfected cells. Expression analysis of human NmU-R2 demonstrated its mRNA to be most highly expressed in central nervous system tissues. Based on these data, we conclude that NmU-R2 is a novel neuromedin U receptor subtype that is likely to mediate central nervous system-specific neuromedin U effects. |
| Databáze: | OpenAIRE |
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