Localization of pyroantimonate-precipitable cation and surface coat anionic binding sites in developing erythrocytic cells and macrophages in normal human bone marrow
| Autor: | Ackerman Ga |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Adult
Antimony Ruthenium red Coat Erythrocytes Histology Cell Golgi Apparatus Bone Marrow Cells Biology Stain Ruthenium Pathology and Forensic Medicine chemistry.chemical_compound Bone Marrow medicine Humans Macrophage Binding site Thorotrast Phospholipids Glycoproteins Inclusion Bodies Ions Binding Sites Staining and Labeling Histocytochemistry Macrophages Cell Differentiation Cell Biology Mitochondria Staining Microscopy Electron medicine.anatomical_structure chemistry Biochemistry Ferritins Biophysics Thorium Dioxide Glycogen |
| Zdroj: | Zeitschrift f�r Zellforschung und mikroskopische Anatomie. 134:153-166 |
| ISSN: | 1432-0878 0302-766X |
| Popis: | The surfaces of developing erythrocytic cells and macrophages have been examined in normal human bone marrow by means of the pyroantimonate-osmium, ruthenium red and Thorotrast techniques for inorganic cations, surface glycoprotein-phospholipid complexes and surface anionic binding sites, respectively. No differences in the degree of surface coat reactivity were noted in the erythrocytic cells at different stages of maturation while pyroantimonate binding to the plasmalemma was not evident developmentally until the final stages of erythrocytic development. Rhopheocytotic invaginations proved to be chemically distinct from the remainder of the cell surface since they did not bind Thorotrast or pyroantimonate and gave more staining with ruthenium red. Pyroantimonate does not bind to the surface of macrophages and the binding of Thorotrast by these cells is less. Macrophage-erythrocytic cell contact zones did not stain with Thorotrast but stained with ruthenium red. The significance of these observations is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink