On the mechanism of cryoprotection by polyethylene oxide
| Autor: | M.G. Shenberg, E.I. Oboznaya, N.S. Pushkar |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Cell Survival
Bone Marrow Cells macromolecular substances Glucosephosphate Dehydrogenase General Biochemistry Genetics and Molecular Biology Cryoprotective Agents Oxygen Consumption Bone Marrow Cryoprotective Agent Leukocytes medicine Humans Dihydrolipoamide Dehydrogenase chemistry.chemical_classification Oxidative metabolism DNA synthesis Chemistry technology industry and agriculture Oxides DNA General Medicine Polyethylene oxide Oxygen uptake Peripheral blood Enzyme medicine.anatomical_structure Biochemistry Bone marrow Polyethylenes General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 13:142-146 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/0011-2240(76)90125-5 |
| Popis: | Bone marrow cells and peripheral blood leukocytes have been exposed to a 15% solution of polyethylene oxide of molecular weight 400 (PEO-400) and the effect on DNA synthesis, oxygen uptake, and the activity of oxidative-reductive enzymes has been studied. All the metabolic activities studied were reduced during exposure to PEO-400, but in each case the effect was completely reversed when the cryoprotective agent was removed. It is suggested that the cryoprotective action of PEO-400 may be linked with its ability not only to dehydrate cells but also to depress oxidative metabolism. |
| Databáze: | OpenAIRE |
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