Treating Cloned Embryos, But Not Donor Cells, with 5-aza-2’-deoxycytidine Enhances the Developmental Competence of Porcine Cloned Embryos
| Autor: | Jiang Zhu, Qing Ran Kong, Hongbin He, Zhonghua Liu, Bing Teng Xie, Yang Zhou, Shichao Liu, Jian Yu Wang, Yan Jun Huan |
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| Rok vydání: | 2013 |
| Předmět: |
DNA (Cytosine-5-)-Methyltransferase 1
Male Homeobox protein NANOG Blastomeres Nuclear Transfer Techniques animal structures Cloning Organism Sus scrofa Down-Regulation Biology Decitabine Embryo development Epigenesis Genetic Embryo Culture Techniques Fetus 5-aza-2’-deoxycytidine Gene expression medicine Animals DNA (Cytosine-5-)-Methyltransferases RNA Messenger Blastocyst Enzyme Inhibitors Cells Cultured Somatic cell nuclear transfer Cloning Regulation of gene expression Pig Gene Expression Regulation Developmental Embryo DNA Methylation Fibroblasts Molecular biology In Vitro Oocyte Maturation Techniques medicine.anatomical_structure embryonic structures Azacitidine Ectogenesis Female Original Article Animal Science and Zoology Reprogramming Abattoirs |
| Zdroj: | The Journal of Reproduction and Development |
| ISSN: | 1348-4400 0916-8818 |
| DOI: | 10.1262/jrd.2013-026 |
| Popis: | The efficiency of cloning by somatic cell nuclear transfer (SCNT) has remained low. In most cloned embryos, epigenetic reprogramming is incomplete, and usually the genome is hypermethylated. The DNA methylation inhibitor 5-aza-2’-deoxycytidine (5-aza-dC) could improve the developmental competence of cow, pig, cat and human SCNT embryos in previous studies. However, the parameters of 5-aza-dC treatment among species are different, and whether 5-aza-dC could enhance the developmental competence of porcine cloned embryos has still not been well studied. Therefore, in this study, we treated porcine fetal fibroblasts (PFF) that then were used as donor nuclei for nuclear transfer or fibroblast-derived reconstructed embryos with 5-aza-dC, and the concentration- and time-dependent effects of 5-aza-dC on porcine cloned embryos were investigated by assessing pseudo-pronucleus formation, developmental potential and pluripotent gene expression of these reconstructed embryos. Our results showed that 5-aza-dC significantly reduced the DNA methylation level in PFF (0 nM vs. 10 nM vs. 25 nM vs. 50 nM, 58.70% vs. 37.37% vs. 45.43% vs. 39.53%, P |
| Databáze: | OpenAIRE |
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