Engineering of Human Corneal Endothelial Cells In Vitro
| Autor: | Yingting Zhu, Sean Tighe, Qin Zhu, Yongsong Liu, Min Hu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Corneal endothelium
endothelium genetic structures Endothelium engineering medicine.medical_treatment Review Corneal Transplantation 03 medical and health sciences 0302 clinical medicine cornea Cornea medicine Humans Endothelial dysfunction Induced pluripotent stem cell Corneal transplantation Cell Proliferation Tissue Engineering business.industry Endothelium Corneal Endothelial Cells progenitor General Medicine medicine.disease Embryonic stem cell eye diseases medicine.anatomical_structure Cancer research 030211 gastroenterology & hepatology sense organs business Reprogramming |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| Popis: | Human corneal endothelial cells are responsible for controlling corneal transparency, however they are notorious for their limited proliferative capability. Thus, damage to these cells may cause irreversible blindness. Currently, the only way to cure blindness caused by corneal endothelial dysfunction is via corneal transplantation of a cadaver donor cornea with healthy corneal endothelium. Due to severe shortage of donor corneas worldwide, it has become paramount to develop human corneal endothelial grafts in vitro that can subsequently be transplanted in humans. Recently, we have reported effective expansion of human corneal endothelial cells by reprogramming the cells into progenitor status through use of p120-Kaiso siRNA knockdown. This new reprogramming approach circumvents the need of using induced pluripotent stem cells or embryonic stem cells. Successful promotion of this technology will encourage scientists to re-think how "contact inhibition" can safely be perturbed to our benefit, i.e., effective engineering of an in vivo-like tissue while successful maintaining the normal phenotype. In this review, we present current advances in reprogramming corneal endothelial cells in vitro, detail the methods to successful engineer human corneal endothelial grafts, and discuss their future clinical applications to cure corneal blindness. |
| Databáze: | OpenAIRE |
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