Structural characterization and immunomodulatory activity of novel polysaccharides from Citrus aurantium Linn. variant amara Engl
| Autor: | Wei Zhu, Chun-Yan Shen, Chao-Yang Zheng, Ming-Qiang Li, Jian-Guo Jiang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Arabinose Immunomodulatory activity Medicine (miscellaneous) Mannose 02 engineering and technology Biology Polysaccharide NF-κB 03 medical and health sciences chemistry.chemical_compound Extracellular TX341-641 Protein kinase A chemistry.chemical_classification Nutrition and Dietetics Kinase Nutrition. Foods and food supply Structure 021001 nanoscience & nanotechnology MAPK 030104 developmental biology chemistry Biochemistry Galactose 0210 nano-technology Citrus aurantium L. var. amara Engl Food Science |
| Zdroj: | Journal of Functional Foods, Vol 35, Iss, Pp 352-362 (2017) |
| ISSN: | 1756-4646 |
| Popis: | Two polysaccharides CAVAP-I and CAVAP-II from Citrus aurantium L. var. amara Engl showed significant structural differences. Average molecular weights of CAVAP-I and CAVAP-II were 10.5 kDa and 4.5 kDa. Triple-helix conformation existed in CAVAP-II rather than CAVAP-I. Arabinose, mannose, glucose and galactose formed main components of CAVAP-I and CAVAP-II at different ratios. (1 → 3,6)-β- d -Gal and (1 → 5)-α- l -Ara composed the backbone of CAVAP-I and CAVAP-II. CAVAP-II displayed greater immune enhancement potential than CAVAP-I. CAVAP-II significantly promoted interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) production, and inducible nitric oxide synthase, IL-6, TNF-α and interleukin-1 beta mRNA expression by activating phosphorylated extracellular signal-regulated kinase, c-Jun N-terminal kinase, P38 and P65. These results indicated that structures were closely related to immunomodulatory activity of polysaccharides. CAVAP-II showed the greatest immunostimulation potential followed by CAVAP-I and CAVAPs by activating mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) pathways. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect