Characterization of a human induced Pluripotent Stem (iPS) cell line (INCABRi002-A) derived from a primary myelofibrosis patient harboring the 5-bp insertion in CALR and the p.W146X mutation in TP53
| Autor: | Ilana Zalcberg, Sylvie Devalle, Rodrigo Madeiro da Costa, Marcelo Reis, Adelmo Daumas, Mariana D'Andrea, Cintia Elisabeth Gomez Limia, Telma Padilha, Stevens K. Rehen, Martín Hernán Bonamino, Bárbara C.R. Monte-Mór, Jaroslaw Sochacki, Cristiana Solza |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Transgene Induced Pluripotent Stem Cells Biology 03 medical and health sciences Cell & Tissue Engineering medicine Humans Myelofibrosis Induced pluripotent stem cell Gene lcsh:QH301-705.5 Aged Science & Technology JAK-STAT signaling pathway General Medicine Cell Biology SOMATIC MUTATIONS medicine.disease biology.organism_classification Sendai virus 030104 developmental biology lcsh:Biology (General) Biotechnology & Applied Microbiology Cell culture Primary Myelofibrosis Mutation Cancer research Tumor Suppressor Protein p53 Reprogramming Life Sciences & Biomedicine Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 33, Iss, Pp 130-134 (2018) |
| Popis: | Primary myelofibrosis (PMF) is a hematological malignancy characterized by activation of the JAK/STAT pathway and risk of leukemic transformation. In this study, we generated an induced Pluripotent Stem (iPS) cell line derived from a 65-year old male PMF patient carrying the 5-pb insertion in the CALR gene (CALRins5) and the c.437 G > A mutation in the TP53 gene (p.W146X). The newly derived PMF3.17 iPS cell line harbors the original mutations and was characterized as bona fide iPS. Resource table Unique stem cell line identifier INCABRi002-A Alternative name(s) of stem cell line PMF3.17 Institution Brazilian National Cancer Institute and D'Or Institute for Research and Education Contact information of distributor Martin Bonamino, PhD, mbonamino@inca.gov.br , Barbara Monte Mor, PhD, barbara.montemor@inca.gov.br , Stevens Rehen, PhD, srehen@lance-ufrj.org Type of cell line iPS cell Origin Human Additional origin info Age: 65 Sex: male Cell source Peripheral blood Clonality Clonal Method of reprogramming Sendai Virus Genetic modification No Type of modification Not applicable Associated disease Primary myelofibrosis Gene CALR gene: CALRins5, c.1154_1155insTTGTC, p.K385 fs*47 TP53 gene: c.437 G > A, p.W146X Method of modification Not applicable Name of transgene or resistance Not applicable Inducible/constitutive system Not applicable Date archived/stock date July 7th, 2015 Cell line repository/bank Not applicable Ethical approval Ethics Committee of the Brazilian National Cancer Institute (INCA) under the number 062/08. Ethics Review Board-competent authority obtained. |
| Databáze: | OpenAIRE |
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