Intra nasal administration of poly-lactic acid microsphere co-encapsulated Yersinia pestis subunits confers protection from pneumonic plague in the mouse
Autor: | Ian D. Spiers, Jim E. Eyles, Gregory J.E. Sharp, H. Oya Alpar, E. Diane Williamson |
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Rok vydání: | 1998 |
Předmět: |
Male
Pneumonic plague Macromolecular Substances Polymers Yersinia pestis Polyesters Mice Inbred Strains Microbiology Mice Antigen medicine Animals Lactic Acid Administration Intranasal Antigens Bacterial Plague General Veterinary General Immunology and Microbiology biology Immunogenicity Public Health Environmental and Occupational Health Yersiniosis biology.organism_classification medicine.disease Enterobacteriaceae Virology Microspheres Immunoglobulin A Infectious Diseases Gastric Mucosa Immunoglobulin G Humoral immunity Molecular Medicine Nasal administration |
Zdroj: | Vaccine. 16:698-707 |
ISSN: | 0264-410X |
DOI: | 10.1016/s0264-410x(97)00249-1 |
Popis: | Equivocal doses of soluble, or high molecular weight poly (lactic acid) microsphere co-encapsulated, F1 and V subunit antigens of Yersinia pestis were used to immunize mice intra-nasally. Animals were dosed on day 1 and 7 with 2.724 micrograms V plus 0.956 micrograms F1. Co-encapsulated antigens induced superior systemic and mucosal immunity in comparison with free F1 and V. All of the mice immunized with soluble antigens died shortly after an aerosol challenge consisting of 1 x 10(5) colony-forming units of plague bacteria. In contrast, 66% of the co-encapsulated subunit vaccinees survived this lethal challenge. Humoral immunity to plague was improved further, resulting in 80% protection from challenge, if a relatively high dose (10 micrograms) of cholera toxin B subunit was added to the microsphere suspension prior to intra-nasal delivery. Significantly, by adding 10 micrograms cholera toxin B subunit to the free antigen solution, a 100% post-challenge survival rate was attained. We conclude that in this animal model of pneumonic plague, intra-nasal administration of microgram quantities of Yersinia pestis subunits confers protective immunity, provided the vaccines are microencapsulated or admixed with a strong mucosal adjuvant, such as the cholera toxin B subunit. |
Databáze: | OpenAIRE |
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