In pursuit of sensitivity: Lessons learned from viral nucleic acid detection and quantification on the Raindance ddPCR platform

Autor:	Samuel Long
Rok vydání:	2022
Předmět:	Viral nucleic acid Cure research Human immunodeficiency virus (HIV) HIV Infections qPCR quantitative (real time) polymerase chain reaction Ct threshold cycle PCR sensitivity medicine.disease_cause HO high occupancy miRNA microRNA HTLV human T-cell leukaemia virus Digital polymerase chain reaction dPCR digital PCR RT-qPCR quantitative reverse transcription (real time) polymerase chain reaction SIV simian immunodeficiency virus COVID-19 Coronavirus disease 2019 Tm melting temperature 0303 health sciences NHP non-human primate cART combination antiretroviral therapy 030302 biochemistry & molecular biology CT Computed Tomography SNP single nucleotide polymorphism STLV-1 simian T cell lymphotropic viruses type 1 Virus FFPE formalin-fixed paraffin-embedded HIV human immunodeficiency virus RT reverse transcriptase RNA Viral Infectious diseases dMIQE Minimum Information for Publication of Quantitative digital PCR experiments Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) HPV human papillomavirus Context (language use) Computational biology LoD limit of detection Biology Real-Time Polymerase Chain Reaction Sensitivity and Specificity SARS-CoV-2 severe acute respiratory syndrome coronavirus 2 Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences medicine Humans STLV-2 simian T cell lymphotropic viruses type 2 SARS severe acute respiratory syndrome ddPCR droplet digital polymerase chain reaction Molecular Biology 030304 developmental biology SARS-CoV-2 SNV single nucleotide variant COVID-19 HIV NGS next generation sequencing DNA PIV parainfluenza virus MGB minor groove binder Infectious disease (medical specialty) Nucleic acid RNA RSV respiratory syncytial virus RT-dPCR reverse transcription digital polymerase chain reaction Digital PCR
Zdroj:	Methods (San Diego, Calif.)
ISSN:	1046-2023
DOI:	10.1016/j.ymeth.2021.04.008
Popis:	Sensitive PCR detection of viral nucleic acids plays a critical role in infectious disease research, diagnosis and monitoring. In the context of SARS-CoV-2 detection, recent reports indicate that digital PCR-based tests are significantly more sensitive than traditional qPCR tests. Numerous factors can influence digital PCR reaction sensitivity. In this review, using a model for human HIV infection and the Raindance ddPCR platform as an example, we describe technical aspects that contribute to sensitive viral signal detection in DNA and RNA from tissue samples, which often harbor viral reservoirs and serve as better predictors of disease outcome and indicators of treatment efficacy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40ffd41efd3a3c73b76e2e0fb9f5b477 https://doi.org/10.1016/j.ymeth.2021.04.008 Zobrazit plný text záznamu Full Text from ScienceDirect