Endogenous indole-3-acetic acid and nitric oxide are required for calcium-mediated alleviation of copper oxide nanoparticles toxicity in wheat seedlings
| Autor: | Vaishali Yadav, Durgesh Kumar Tripathi, Weijun Zhou, Naleeni Ramawat, Skhawat Ali Gill, Rafaqat A. Gill, Namira Arif, Vijay Pratap Singh, Devendra Kumar Chauhan |
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| Rok vydání: | 2021 |
| Předmět: |
Physiology
chemistry.chemical_element Plant Science Calcium medicine.disease_cause Nitric Oxide Antioxidants Nitric oxide Lipid peroxidation chemistry.chemical_compound Genetics medicine Triticum Indoleacetic Acids food and beverages Cell Biology General Medicine Glutathione Hydrogen Peroxide Malondialdehyde Oxidative Stress chemistry Biochemistry Seedlings Toxicity Nanoparticles Indole-3-acetic acid Oxidative stress Copper |
| Zdroj: | Physiologia plantarumREFERENCES. 173(4) |
| ISSN: | 1399-3054 |
| Popis: | The action of nanoparticles is increasingly being studied in recent years to minimize their toxic impacts. Besides this, efforts are also being made to minimize their toxicity in crop plants by using various chemicals, i.e. nutrients, donors of signalling molecules, plant hormones, etc. However, associated alleviatory mechanisms are still not well known. Therefore, in the present study, we have investigated the toxicity of CuONPs and its mitigation by exogenously applied calcium (Ca). The focus was on whether indole-3-acetic acid (IAA) or endogenous nitric oxide (NO) has any role in accomplishing this task. CuONPs declined wheat growth due to increased accumulation of Cu and oxidative stress markers such as superoxide radicals, hydrogen peroxide and lipid peroxidation (malondialdehyde) and it was also accompanied by a decline in endogenous NO. CuONPs also altered the redox status of ascorbate and glutathione by inhibiting the activity of their regenerating enzymes. This collectively leads to cell death in wheat seedlings. However, exogenous supplementation of Ca mitigated toxic effects of CuONPs by reducing the excess accumulation of Cu, which caused remarkable enhancement in growth, protein contents, photosynthetic pigments, and endogenous nitric oxide; altogether protecting wheat roots from cell death. Interestingly, addition of 2,3,5-triiodobenzoic acid (TIBA) further increased CuONPs toxicity even in the presence of Ca, but the addition of IAA rescued this effect of TIBA. These results clearly show that Ca mitigates CuONPs toxicity in wheat seedlings by involving IAA. Further, the results also showed that endogenous NO has a positive and indispensable role in Ca-mediated mitigation of CuONPs toxicity in wheat seedlings. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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