A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast
Autor: | Elvira Manjón, Yolanda Sánchez, Tomás Edreira, Rubén Celador |
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Přispěvatelé: | Junta de Castilla y León, Universidad de Salamanca, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Meteorología (España) |
Rok vydání: | 2020 |
Předmět: |
MAPK/ERK pathway
QH301-705.5 Science cytokinesis Ingression Rho1 GEF General Biochemistry Genetics and Molecular Biology Constriction Cell wall stress Abscission Schizosaccharomyces Guanine Nucleotide Exchange Factors Biology (General) General Immunology and Microbiology Chemistry General Neuroscience Wild type General Medicine Actomyosin Cell Biology Phenotype fission yeast Cell biology Medicine cell wall Schizosaccharomyces pombe Proteins actomyosin ring constriction Mitogen-Activated Protein Kinases Cytokinesis Research Article S. pombe |
Zdroj: | eLife Digital.CSIC. Repositorio Institucional del CSIC instname eLife, Vol 9 (2020) |
ISSN: | 2050-084X |
Popis: | In fission yeast, the septation initiation network (SIN) ensures temporal coordination between actomyosin ring (CAR) constriction with membrane ingression and septum synthesis. However, questions remain about CAR regulation under stress conditions. We show that Rgf1p (Rho1p GEF), participates in a delay of cytokinesis under cell wall stress (blankophor, BP). BP did not interfere with CAR assembly or the rate of CAR constriction, but did delay the onset of constriction in the wild type cells but not in the rgf1¿ cells. This delay was also abolished in the absence of Pmk1p, the MAPK of the cell integrity pathway (CIP), leading to premature abscission and a multi-septated phenotype. Moreover, cytokinesis delay correlates with maintained SIN signaling and depends on the SIN to be achieved. Thus, we propose that the CIP participates in a checkpoint, capable of triggering a CAR constriction delay through the SIN pathway to ensure that cytokinesis terminates successfully. T Edreira and E Manjon were financially supported by a contract obtained through the Regional Government of Castile and Leon, co-financed by the European Social Fund. R Celador was financially supported by a collaboration grant from the University of Salamanca. Funding sources: MEIC, Spain (BFU2017-84508-P; Regional Government of Castile and Leon [SA073U14]). Funding for open access charge: MEIC. |
Databáze: | OpenAIRE |
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