Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays
| Autor: | Mizanur Khondoker, Andrew R. Mount, Alan J. Ross, Peter Ghazal, Jason Crain, Holger Schulze, Till T. Bachmann, Anthony J. Walton, Chaker Tlili, Ilenia Ciani, Gerard Giraud, Colin Campbell, Jonathan G. Terry, Stuart W. J. Ember |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science Cytomegalovirus Nanotechnology Hepacivirus Article Catalysis lcsh:Chemistry Inorganic Chemistry Nucleic acid thermodynamics Transduction (genetics) DNA Microarray Microscopy Quantum Dots Humans Physical and Theoretical Chemistry lcsh:QH301-705.5 Lifetime Multiplexing Molecular Biology Spectroscopy Oligonucleotide Array Sequence Analysis Fluorescence Lifetime Imaging Microscopy Quantum Dots (QD) Fluorescence Lifetime Imaging Microscopy (FLIM) business.industry Organic Chemistry Nucleic Acid Hybridization DNA General Medicine Fluorescence Computer Science Applications Microscopy Fluorescence lcsh:Biology (General) lcsh:QD1-999 Quantum dot DNA Viral RNA Viral Optoelectronics Contrast ratio DNA microarray business |
| Zdroj: | Giraud, G, Schulze, H, Bachmann, T T, Campbell, C J, Mount, A R, Ghazal, P, Khondoker, M R, Ross, A J, Ember, S W J, Ciani, I, Tlili, C, Walton, A J, Terry, J G & Crain, J 2009, ' Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays ', International Journal of Molecular Sciences, vol. 10, no. 4, pp. 1930-1941 . https://doi.org/10.3390/ijms10041930 International Journal of Molecular Sciences, Vol 10, Iss 4, Pp 1930-1941 (2009) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 10; Issue 4; Pages: 1930-1941 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms10041930 |
| Popis: | Quantum dot (QD) labeling combined with fluorescence lifetime imaging\ud microscopy is proposed as a powerful transduction technique for the detection of DNA\ud hybridization events. Fluorescence lifetime analysis of DNA microarray spots of\ud hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared\ud to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to\ud the spot microenvironment. Additionally, time gated detection was shown to improve the\ud microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single\ud excitation-detection readout channel. |
| Databáze: | OpenAIRE |
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