Δ12-fatty acid desaturase is involved in growth at low temperature in yeast Yarrowia lipolytica
| Autor: | Satoshi Tezaki, Satoshi Kobayashi, Hirofumi Yoshikawa, Ryo Iwama, Hiroyuki Horiuchi, Yuh Shiwa, Akinori Ohta, Ryouichi Fukuda |
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| Rok vydání: | 2017 |
| Předmět: |
Fatty Acid Desaturases
0301 basic medicine chemistry.chemical_classification biology 030106 microbiology Temperature Biophysics Yarrowia Fatty acid Cell Biology biology.organism_classification Biochemistry Yeast 03 medical and health sciences Oleic acid chemistry.chemical_compound Fatty acid desaturase chemistry Transcription (biology) biology.protein Northern blot Molecular Biology Incubation |
| Zdroj: | Biochemical and Biophysical Research Communications. 488:165-170 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2017.05.028 |
| Popis: | We investigated the role of FAD2, which was predicted to encode a fatty acid desaturase of the n-alkane-assimilating yeast Yarrowia lipolytica. Northern blot analysis suggested that FAD2 transcription was upregulated at low temperature or in the presence of n-alkanes or oleic acid. The FAD2 deletion mutant grew as well as the wild-type strain on glucose, n-alkanes, or oleic acid at 30 °C, but grew at a slower rate at 12 °C, when compared to the wild-type strain. The growth of the FAD2 deletion mutant at 12 °C was restored by the addition of 18:2, but not 18:1, fatty acids. The amount of 18:2 fatty acid in the wild-type strain was increased by the incubation at 12 °C and in the presence of n-octadecane. In contrast, 18:2 fatty acid was not detected in the deletion mutant of FAD2, confirming that FAD2 encodes the Δ12-fatty acid desaturase. These results suggest that Δ12-fatty acid desaturase is involved in the growth of Y. lipolytica at low temperature. |
| Databáze: | OpenAIRE |
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