Current status of oxacillin-susceptible mecA-positive Staphylococcus aureus infection in Shanghai, China: A multicenter study
| Autor: | Li-Hua He, Tianming Li, Wen-Xia Zhang, Yun Liu, Yin-Juan Guo, Xing Wang, Min Li, Jie Jiang, Wen-Juan Wu, Rong Tang, Jun-Lan Liu, Jin Tang, Jiang Hu, Ni Zhong |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.disease_cause 0302 clinical medicine Drug Resistance Multiple Bacterial polycyclic compounds Immunology and Allergy Shanghai china 030212 general & internal medicine Child Oxacillin Broth microdilution General Medicine Staphylococcal Infections VITEK 2 QR1-502 Anti-Bacterial Agents Infectious Diseases Staphylococcus aureus medicine.drug Methicillin-Resistant Staphylococcus aureus Microbiology (medical) China Genotype 030106 microbiology Microbial Sensitivity Tests OS-MRSA Microbiology Cefoxitin 03 medical and health sciences Bacterial Proteins medicine Humans Penicillin-Binding Proteins Typing Cities Antimicrobial susceptibility tests General Immunology and Microbiology business.industry SCCmec biochemical phenomena metabolism and nutrition bacterial infections and mycoses Multicenter study Mutation bacteria Multilocus sequence typing business Multilocus Sequence Typing |
| Zdroj: | Journal of Microbiology, Immunology and Infection, Vol 54, Iss 6, Pp 1070-1077 (2021) |
| ISSN: | 1684-1182 |
| DOI: | 10.1016/j.jmii.2020.07.021 |
| Popis: | Background Oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) represents an important issue, as its oxacillin susceptibility has contributed to misidentification by conventional susceptibility tests and consequently potential therapeutic failure, but limited data on the current status of OS-MRSA infection in Chinese hospitals are available. Methods This multicenter study performed a battery of susceptibility tests and diagnostic tests for 956 S. aureus isolates from 10 hospitals, including automated susceptibility testing on VITEK 2, broth microdilution, disk diffusion, and detection of PBB2a, mecA gene and mecC gene. For all identified OS-MRSA, multi-locus sequence typing (MLST), together with spa typing, SCCmec typing and PVL detecting, was carried out. Results OS-MRSA, most of which were from pediatric inpatients, represented 1.8% (17/956) of total isolates. Of these 17 OS-MRSA, 10 were ST59, followed by ST965 (3/17), and 11 carried SCCmec type IV, while 5 carried SCCmec type V, but only one was Panton–Valentine leucocidin (PVL)-positive, also, 16 had one or two point mutations within mecA promoter. OS-MRSA had inducible oxacillin resistance and significantly lower MDR (Multi-Drug Resistant) rate. We observed that the VITEK 2 system exhibited some deficiency in OS-MRSA detection, whereas cefoxitin disk diffusion was shown to be a reliable and cost-saving alternative and should be supplemented in detecting S. aureus with borderline oxacillin susceptible MICs. Conclusion This study has characterized phenotypically and molecularly OS-MRSA in China, and provided insights into more effective management of OS-MRSA. |
| Databáze: | OpenAIRE |
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