Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt
| Autor: | Fatma I. El Hofy, Herbert Tomaso, Stefan Monecke, Heinrich Neubauer, Helmut Hotzel, Ashraf Awad Abd El-Tawab, Wedad Ahmed |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
medicine.drug_class Antibiotics lcsh:Medicine Biology medicine.disease_cause mastitis Microbiology Antibiotic resistance enterococci resistance gene medicine Escherichia coli Immunology and Allergy Molecular Biology Gene General Immunology and Microbiology Communication Broth microdilution lcsh:R DNA microarray biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification Mastitis Infectious Diseases Enterococcus Egypt |
| Zdroj: | Pathogens Pathogens, Vol 10, Iss 97, p 97 (2021) |
| ISSN: | 2076-0817 |
| Popis: | This study aimed to investigate the prevalence and antimicrobial resistance of enterococci- and ESBL-producing E. coli isolated from milk of bovine mastitis cases in Egypt. Fifty milk samples of dairy animals were collected from localities in the Nile Delta region of Egypt. Isolates were identified using MALDI-TOF MS, and antibiotic susceptibility testing was performed by the broth microdilution method. PCR amplifications were carried out, targeting resistance-associated genes. Seventeen Enterococcus isolates and eight coliform isolates could be cultivated. Vancomycin resistance rate was high in Ent. faecalis. The VITEK 2 system confirmed all E. coli isolates as ESBL-producing. All Ent. faecalis isolates harbored erm(B), tetL and aac-aphD genes. The vanA gene was detected in Ent. faecalis isolate, vanB was found in other Enterococcus, while one isolate of E. casseliflavus exhibited the vanA gene. E. coli isolates exhibited high prevalence of erm(B) and tetL. E. coli isolates were analyzed by DNA microarray analysis. Four isolates were determined by O-serotyping as O8 (n = 1), O86 (n = 2) and O157 (n = 1). H-serotyping resulted in H11, H12, H21 (two isolates each) and one was of H16 type. Different virulence-associated genes were detected in E. coli isolates including lpfA, astA, celB, cmahemL, intI1 and intI2, and the iroN gene was identified by DNA microarray analysis. |
| Databáze: | OpenAIRE |
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