Enrichment of bacterial strains for the biodegradation of diclofenac and carbamazepine from activated sludge

Autor: Bessa, V. S., Moreira, I. S., Tiritan, M. E., Castro, P. M. L.
Přispěvatelé: CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Veritati - Repositório Institucional da Universidade Católica Portuguesa
Rok vydání: 2017
Předmět:
Environmental contaminant
0208 environmental biotechnology
Environmental pollution
Wastewater treatment
genetic analysis
02 engineering and technology
010501 environmental sciences
biodegradation
01 natural sciences
Rhizobium sp. c12
activated sludge
pollutant removal
Starkeya
Food science
Bacteria (microorganisms)
Waste Management and Disposal
16S rRNA gene sequencing
biology
Chemistry
drug
Environmental pollutions
Contamination
bacterium
6. Clean water
3. Good health
Carbamazepine
Wastewater
Biodegradation
Pharmaceuticals
environmental fate
Sewage treatment
Selective enrichment
feeding
biotechnology
medicine.drug
Diclofenac
Brevibacterium sp
Microbiology
Biomaterials
medicine
wastewater treatment plant
0105 earth and related environmental sciences
Biotechnological applications
Activated sludge process
Drug products
Municipal wastewater treatment plants
biology.organism_classification
Amides
020801 environmental engineering
Activated sludge
13. Climate action
RNA
Bacteria
Zdroj: International Biodeterioration & Biodegradation
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
ISSN: 0964-8305
DOI: 10.1016/j.ibiod.2017.02.008
Popis: Carbamazepine and diclofenac have been pointed out as important markers for environmental pollution by pharmaceuticals. This study reports on the isolation of bacterial strains capable to degrade these micropollutants from activated sludge of a municipal wastewater treatment plant (WWTP). After selective enrichments, one strain able to degrade diclofenac and two strains able to degrade carbamazepine were isolated. The strains were identified by 16S rRNA gene sequencing. Strain Brevibacterium sp. D4 was able to biodegrade 35% of 10 mg L−1 of diclofenac as a sole carbon source; periodic feeding with acetate as a supplementary carbon source resulted in enhancing biodegradation to levels up to 90%, with a concomitant increase of the biodegradation rate. Strains Starkeya sp. C11 and Rhizobium sp. C12 were able to biodegrade 30% of 10 mg L−1 of carbamazepine as a sole carbon source; supplementation with acetate did not improve the biodegradation of carbamazepine by these strains. The activated sludge harboured bacteria capable to degrade the two top priority environmental contaminants and may be potentially useful for biotechnological applications. © 2017 Elsevier V.S. Bessa and I.S. Moreira wish to acknowledge research grant from Funda??o para a Ci?ncia e a Tecnologia (FCT), Portugal (Ref. SFRH/BD/90146/2012 and SFRH/BPD/87251/2012, respectively) and Fundo Social Europeu (Programa Operacional Potencial Humano (POPH), Quadro de Refer?ncia Estrat?gico Nacional (QREN)). This work was supported by FCT through the projects PTDC/EBB-EBI/111699/2009, UID/Multi/04423/2013, PEst-OE/EQB/LA0016/2013 and PHARMADRUGS-CESPU-2014.
Databáze: OpenAIRE
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