Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XF(e)96 Extracellular Flux Analyzer
| Autor: | Gerhard Engelbrecht, S. Höss, H. Miller, Hendrika Fourie, M. Daneel, G. du Preez, Victor Wepener, Cristian Ricci, M. Zouhar |
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| Přispěvatelé: | 21621217 - Du Preez, Gerhard Cornelis, 10148620 - Fourie, Hendrika, 22135189 - Miller, Hayley Christy, 29790514 - Ricci, Cristian, 24137472 - Engelbrecht, Gerhard, 12579769 - Wepener, Victor |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Medicine
010501 environmental sciences Toxicology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound In vivo Extracellular Caenorhabditis elegans lcsh:Science 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Multidisciplinary biology Chemistry lcsh:R High-throughput screening Environmental exposure biology.organism_classification Dose–response relationship Biochemistry Toxicity lcsh:Q Growth inhibition Flux (metabolism) |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) |
| Popis: | Caenorhabditis elegans presents functioning, biologically relevant phenotypes and is frequently used as a bioindicator of toxicity. However, most C. elegans in vivo effect-assessment methods are laborious and time consuming. Therefore, we developed a novel method to measure the oxygen consumption rate of C. elegans as a sublethal endpoint of toxicity. This protocol was tested by exposing 50 larval stage one C. elegans individuals for 48 h (at 20 °C) to different concentrations of two toxicants i.e. benzylcetyldimethylammonium chloride (BAC-C16) and cadmium (Cd). Following exposures, the oxygen consumption rate of the C. elegans individuals were measured using the high-throughput functionality of the Seahorse XFe96 Extracellular Flux Analyzer. Dose-response curves for BAC-C16 (R2 = 0.93; P = 0.001) and Cd (R2 = 0.98; P = 0.001) were created. Furthermore, a strong, positive correlation was evidenced between C. elegans oxygen consumption rate and a commonly used, ecologically relevant endpoint of toxicity (growth inhibition) for BAC-C16 (R2 = 0.93; P = 0.0001) and Cd (R2 = 0.91; P = 0.0001). The data presented in this study show that C. elegans oxygen consumption rate can be used as a promising functional measurement of toxicity. |
| Databáze: | OpenAIRE |
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