TMEM119 as a specific marker of microglia reaction in traumatic brain injury in postmortem examination

Autor: Stefanie Trella, Camelia-Maria Monoranu, Luitpold Distel, Simone Bohnert, Michael Bohnert, Benjamin Ondruschka, Anja Seiffert
Rok vydání: 2020
Předmět:
Male
Pathology
0302 clinical medicine
Cerebrospinal fluid
Cerebellum
Pons
Cortex (anatomy)
Brain Injuries
Traumatic
Receptors
Immunologic
Child
Forensic Pathology
Aged
80 and over
0303 health sciences
Membrane Glycoproteins
Microglia
Human brain
Middle Aged
Immunohistochemistry
White Matter
medicine.anatomical_structure
Child
Preschool
Female
Original Article
Autopsy
Immunocytochemistry
Adult
medicine.medical_specialty
Adolescent
Receptors
CCR2
Traumatic brain injury
Pathology and Forensic Medicine
White matter
Young Adult
03 medical and health sciences
medicine
Humans
ddc:610
Neuroinflammation
Aged
030304 developmental biology
Forensic neuropathology
business.industry
Membrane Proteins
Biomarker
medicine.disease
Forensic neurotraumatology
business
Biomarkers
030217 neurology & neurosurgery
Zdroj: International Journal of Legal Medicine
ISSN: 1437-1596
0937-9827
DOI: 10.1007/s00414-020-02384-z
Popis: The aim of the present study was a refined analysis of neuroinflammation including TMEM119 as a useful microglia-specific marker in forensic assessments of traumatic causes of death, e.g., traumatic brain injury (TBI). Human brain tissue samples were obtained from autopsies and divided into cases with lethal TBI (n = 25) and subdivided into three groups according to their trauma survival time and compared with an age-, gender-, and postmortem interval-matched cohort of sudden cardiovascular fatalities as controls (n = 23). Brain tissue samples next to cortex contusions and surrounding white matter as well as samples of the ipsilateral uninjured brain stem and cerebellum were collected and stained immunohistochemically with antibodies against TMEM119, CD206, and CCR2. We could document the highest number of TMEM119-positive cells in acute TBI death with highly significant differences to the control numbers. CCR2-positive monocytes showed a significantly higher cell count in the cortex samples of TBI cases than in the controls with an increasing number of immunopositive cells over time. The number of CD206-positive M2 microglial cells increased survival time-dependent. After 3 days of survival, the cell number increased significantly in all four regions investigated compared with controls. In sum, we validate a specific and robustly expressed as well as fast reacting microglia marker, TMEM119, which distinguishes microglia from resident and infiltrating macrophages and thus offers a great potential for the estimation of the minimum survival time after TBI. Electronic supplementary material The online version of this article (10.1007/s00414-020-02384-z) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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