Phagocytosing neutrophils down-regulate the expression of chemokine receptors CXCR1 and CXCR2
| Autor: | Valery Savitskiy, Olga V. Maslakova, Yuri V Chaly, Nikolai N. Voitenok, Tatyana Doroshenko, Anna Portyanko, I. V. Gorudko |
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| Rok vydání: | 2002 |
| Předmět: |
Phagocyte
Neutrophils Phagocytosis media_common.quotation_subject Immunology Down-Regulation Saccharomyces cerevisiae Granulocyte Biology In Vitro Techniques Biochemistry Receptors Interleukin-8B Receptors Interleukin-8A Chemokine receptor Cytosol medicine Humans CXC chemokine receptors Receptor Internalization media_common Microscopy Confocal Chemotaxis Cell Biology Hematology Flow Cytometry Cell biology Chemotaxis Leukocyte medicine.anatomical_structure Calcium |
| Zdroj: | Blood. 100(7) |
| ISSN: | 0006-4971 |
| Popis: | CXC chemokines play a central role in regulation of neutrophil activation and chemotaxis. Because the chemotactic responses of neutrophils are impaired after phagocytosis, we explored the effect of phagocytic stimuli on the expression of interleukin-8 (IL-8) receptors, CXCR1 and CXCR2, in human neutrophils. After phagocytosis of opsonized yeast, the expression of CXCR1 and CXCR2 was substantially down-regulated and was accompanied by reduced Ca++responses to corresponding ligands, IL-8 and neutrophil-activating peptide–2 (NAP-2). The levels of CXCR1 and CXCR2 mRNA were constant during phagocytic stimulation of neutrophils. Confocal microscopy revealed that CXCR reduction was not via internalization. Metalloproteinase inhibitor, 1,10-phenantroline, prevented the reduction of CXCRs induced by phagocytosis, indicating that proteolytic degradation may be responsible for down-regulation. These observations suggest that down-regulation of CXCR expression may substantially reduce the responsiveness of phagocytosing neutrophils to CXC chemokines. |
| Databáze: | OpenAIRE |
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