Reviving Phage Therapy for the Treatment of Cholera
| Autor: | Muhammad Ali Shah, Habib Bukhari, Joan Colom, Brendan W. Wren, Paul A. Barrow, Jingliang Su, Banwarilal Sarkar, Robert J. Atterbury, Abiyad Baig, Jenny M Ritchie, S. G. Bhandare |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Phage therapy medicine.drug_class medicine.medical_treatment 030106 microbiology Antibiotics Drug resistance medicine.disease_cause El Tor Article Microbiology Bacteriophage 03 medical and health sciences Cholera medicine Immunology and Allergy Animals Bacteriophages Phage Therapy Vibrio cholerae biology business.industry Outbreak medicine.disease biology.organism_classification Bacterial Load Intestines Disease Models Animal 030104 developmental biology Infectious Diseases Treatment Outcome Rabbits business |
| Zdroj: | J Infect Dis |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | Cholera remains a major risk in developing countries, particularly after natural or man-made disasters. Vibrio cholerae El Tor is the most important cause of these outbreaks, and is becoming increasingly resistant to antibiotics, so alternative therapies are urgently needed. In this study, a single bacteriophage, Phi_1, was used to control cholera prophylactically and therapeutically in an infant rabbit model. In both cases, phage-treated animals showed no clinical signs of disease, compared with 69% of untreated control animals. Bacterial counts in the intestines of phage-treated animals were reduced by up to 4 log(10) colony-forming units/g. There was evidence of phage multiplication only in animals that received a V. cholerae challenge. No phage-resistant bacterial mutants were isolated from the animals, despite extensive searching. This is the first evidence that a single phage could be effective in the treatment of cholera, without detectable levels of resistance. Clinical trials in human patients should be considered. |
| Databáze: | OpenAIRE |
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