Spatial control of active CDC-42 during collective migration of hypodermal cells inCaenorhabditis elegans
| Autor: | Germain Lacoste-Caron, Sarah Jenna, Emmanuel Martin, Karim Hamiche, Marie-Hélène Ouellette |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Leading edge Green Fluorescent Proteins Cell Cycle Proteins CDC42 Epithelial cell migration Models Biological Cell junction Article 03 medical and health sciences Subcutaneous Tissue Cell Movement GTP-Binding Proteins Genetics Animals Pseudopodia Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Actin biology Cell Biology General Medicine biology.organism_classification Actins Cell Compartmentation Cell biology Intercellular Junctions 030104 developmental biology Cdc42 GTP-Binding Protein Cell Surface Extensions |
| Zdroj: | Journal of Molecular Cell Biology. 8:313-327 |
| ISSN: | 1759-4685 1674-2788 |
| DOI: | 10.1093/jmcb/mjv062 |
| Popis: | Collective epithelial cell migration requires the maintenance of cell–cell junctions while enabling the generation of actin-rich protrusions at the leading edge of migrating cells. Ventral enclosure of Caenorhabditis elegans embryos depends on the collective migration of anterior-positioned leading hypodermal cells towards the ventral midline where they form new junctions with their contralateral neighbours. In this study, we characterized the zygotic function of RGA-7/SPV-1, a CDC-42/Cdc42 and RHO-1/RhoA-specific Rho GTPase-activating protein, which controls the formation of actin-rich protrusions at the leading edge of leading hypodermal cells and the formation of new junctions between contralateral cells. We show that RGA-7 controls these processes in an antagonistic manner with the CDC-42′s effector WSP-1/N-WASP and the CDC-42-binding proteins TOCA-1/2/TOCA1. RGA-7 is recruited to spatially distinct locations at junctions between adjacent leading cells, where it promotes the accumulation of clusters of activated CDC-42. It also inhibits the spreading of these clusters towards the leading edge of the junctions and regulates their accumulation and distribution at new junctions formed between contralateral leading cells. Our study suggests that RGA-7 controls collective migration and junction formation between epithelial cells by spatially restricting active CDC-42 within cell–cell junctions. |
| Databáze: | OpenAIRE |
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