Resazurin assay of radiation response in cultured cells
| Autor: | John Carey, E O'Sullivan, F N van Pelt, T. Conere, Shailendra Anoopkumar-Dukie, Ashley Allshire |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty Oxazines Sensitivity and Specificity chemistry.chemical_compound medicine Humans Radiology Nuclear Medicine and imaging Tumor Stem Cell Assay chemistry.chemical_classification Chromatography DNA synthesis Resazurin General Medicine DNA chemistry Bromodeoxyuridine Xanthenes Cell culture Oxygen enhancement ratio Indicators and Reagents Radiation response HeLa Cells |
| Zdroj: | The British journal of radiology. 78(934) |
| ISSN: | 0007-1285 |
| Popis: | We describe use of resazurin reduction for measurement of cell response to irradiation as a simple and non-destructive assay that complements the conventional colony forming assay and can readily be applied to both adherent and non-adherent cell cultures. The resazurin method yields data comparable with the colony forming assay as well as to assay of DNA synthesis (BrdU incorporation), giving an OER (oxygen enhancement ratio) of 2.5 at 60% isoeffect level versus 3.1 for the colony forming assay. Intraday and interday precisions for the resazurin assay were 4.1% and 5.2%, respectively. |
| Databáze: | OpenAIRE |
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