Expression and purification of polyhistidine-tagged firefly luciferase in insect cells
| Autor: | Patrik O. Michel, Tuula K. Torkkeli, Christian Oker-Blom, Matti Karp |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
aviation
Recombinant Fusion Proteins Bioengineering Moths Protein Engineering Applied Microbiology and Biotechnology chemistry.chemical_compound Affinity chromatography Photinus pyralis Animals Luciferase Histidine Polyhistidine-tag Luciferases biology Expanded bed adsorption General Medicine biology.organism_classification Fusion protein Molecular biology Coleoptera aviation.aircraft_model chemistry Biochemistry Light emission Lampyridae Peptides Biotechnology |
| Zdroj: | Michel, P, Torkkeli, T, Karp, M & Oker-Blom, C 2001, ' Expression and purification of polyhistidine-tagged firefly luciferase in insect cells : A potential alternative for process scale-up ', Journal of Biotechnology, vol. 85, no. 1, pp. 49-56 . https://doi.org/10.1016/S0168-1656(00)00377-1 |
| ISSN: | 1873-4863 0168-1656 |
| Popis: | The coleopteran firefly, Photinus pyralis, luciferase was produced in lepidopteran Trichoplusia ni insect cells using a baculovirus expression vector. The recombinant protein was equipped with a polyhistidine affinity tag at the carboxyl terminus and purified by immobilized metal-ion affinity chromatography in combination with an expanded bed adsorption system. This approach enabled an efficient, one-step purification protocol of a genetically modified luciferase with properties similar to those of the authentic counterpart. According to light emission measurements, the final yield of highly purified protein was 23 mg l−1 of cell culture. In addition, no specific interaction of interfering substances, such as, ATP, adenylate kinase, nucleoside diphosphokinase, as well as, creatine kinase of the final preparation were identified. Together, the results presented here clearly show that the baculovirus expression system in combination with immobilized metal-ion affinity chromatography is a potential strategy for process scale-up of polyhistidine tagged insect luciferase. |
| Databáze: | OpenAIRE |
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