Metastatic-niche labelling reveals parenchymal cells with stem features
| Autor: | Anne Weston, Giulia Matacchione, Antranik Mavousian, Lucy M. Collinson, Emma Nolan, Ilaria Malanchi, Ivana Kurelac, Probir Chakravarty, Luigi Ombrato, Alessandro Ori, Stuart Horswell, Victoria L. Bridgeman, Estela Gonzalez-Gualda, Valerie Speirs, Joo-Hyeon Lee, Ivonne Heinze, Ehab Husain, Joanna Kirkpatrick |
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| Přispěvatelé: | Ombrato L., Nolan E., Kurelac I., Mavousian A., Bridgeman V.L., Heinze I., Chakravarty P., Horswell S., Gonzalez-Gualda E., Matacchione G., Weston A., Kirkpatrick J., Husain E., Speirs V., Collinson L., Ori A., Lee J.-H., Malanchi I., Lee, Joo [0000-0002-7364-6422], Apollo - University of Cambridge Repository |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Cell type Lung Neoplasms Neutrophils Breast Neoplasms Biology Article Metastasis Mice 03 medical and health sciences 0302 clinical medicine Parenchyma Tumor Microenvironment medicine Animals Humans Cell Lineage Neoplasm Metastasis Stem Cell Niche Parenchymal Tissue Tumor microenvironment Multidisciplinary Staining and Labeling Cell Differentiation Epithelial Cells medicine.disease Phenotype Metastatic breast cancer Coculture Techniques 3. Good health Organoids Luminescent Proteins 030104 developmental biology Cell Tracking 030220 oncology & carcinogenesis Cancer cell Neoplastic Stem Cells Cancer research Female metastatic-niche breast cancer tumor microenvironment lung metastases Ex vivo |
| Zdroj: | Nature |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/s41586-019-1487-6 |
| Popis: | Direct investigation of the early cellular changes induced by metastatic cells within the surrounding tissue remains a challenge. Here we present a system in which metastatic cancer cells release a cell-penetrating fluorescent protein, which is taken up by neighbouring cells and enables spatial identification of the local metastatic cellular environment. Using this system, tissue cells with low representation in the metastatic niche can be identified and characterized within the bulk tissue. To highlight its potential, we applied this strategy to study the cellular environment of metastatic breast cancer cells in the lung. We report the presence of cancer-associated parenchymal cells, which exhibit stem-cell-like features, expression of lung progenitor markers, multi-lineage differentiation potential and self-renewal activity. In ex vivo assays, lung epithelial cells acquire a cancer-associated parenchymal-cell-like phenotype when co-cultured with cancer cells and support their growth. These results highlight the potential of this method as a platform for new discoveries. A cell-penetrating fluorescent marker is used to label cells in the metastatic tumour microenvironment, revealing a variety of cell types including parenchymal cells with lung stem-cell characteristics. |
| Databáze: | OpenAIRE |
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