Tertiary contacts in alpha-lactalbumin at pH 7 and pH 2: a molecular dynamics study
| Autor: | Naiqi Li, Lynne Reed Murphy, Jean Baum, Ronald M. Levy |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Lactalbumin
Models Molecular Circular dichroism Chemistry Hydrogen bond General Medicine Hydrogen-Ion Concentration Electrostatics Molten globule Protein tertiary structure Protein Structure Tertiary Crystallography Molecular dynamics Structural Biology Computer Simulation Molecular Biology Protein secondary structure |
| Zdroj: | Journal of biomolecular structuredynamics. 16(2) |
| ISSN: | 0739-1102 |
| Popis: | Molecular dynamics simulations of alpha-lactalbumin were performed under conditions of neutral pH and low pH in order to study the acid-induced molten globule state. Through the use of experimental techniques such as NMR and CD spectroscopy, molten globules have been characterized as being compact intermediates with secondary structure similar to that of the native protein but with tertiary structure that is disordered. The detailed structure of the molten globule state is unknown, however. Through the use of computer simulations we can study the structural changes which occur upon lowering pH. The simulations presented here differ from previous unfolding simulations in two important ways: the electrostatic interactions are treated more accurately than ever before, and artificially high temperatures are not used to force the protein to unfold. Simulations of 880 psec each were run at pH 7 (control simulation) and pH 2. We concentrate on the interesting changes in the tertiary interactions within the protein with lowering of pH. In particular, there is a loss of native tertiary contacts in the beta domain and interdomain region, and a large decrease in interdomain hydrogen bonds. |
| Databáze: | OpenAIRE |
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