A facile and efficient single-step approach for the fabrication of vancomycin functionalized polymer-based monolith as chiral stationary phase for nano-liquid chromatography
| Autor: | Dongsheng Xu, Zhengjin Jiang, Elena Sánchez-López, Salvatore Fanali, Qiqin Wang, María Luisa Marina, Huikai Shao, Rongying Luo |
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| Přispěvatelé: | Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Fabrication
Capillary action Polymers 02 engineering and technology Methacrylate 01 natural sciences Biochemistry Analytical Chemistry Polymerization Enantioseparation Organic polymeric monolith chemistry.chemical_compound Vancomycin Nanotechnology Nano-LC Monolith Carteolol Chromatography High Pressure Liquid geography Functionalized polymer Chromatography Reverse-Phase geography.geographical_feature_category Chromatography Dimethyl sulfoxide 010401 analytical chemistry Organic Chemistry Reproducibility of Results Stereoisomerism General Medicine Química 021001 nanoscience & nanotechnology Silicon Dioxide 0104 chemical sciences Thalidomide Chemistry chemistry Chemical engineering Pharmaceutical Preparations Methacrylates Methanol 0210 nano-technology Colchicine Derivative (chemistry) Isocyanates |
| Zdroj: | e_Buah Biblioteca Digital Universidad de Alcalá instname |
| Popis: | A facile single-step preparation strategy for fabricating vancomycin functionalized organic polymer based monolith within 100 mu m fused-silica capillary was developed. The synthetic chiral functional monomer, i.e 2-isocyanatoethyl methacrylate (ICNEML) derivative of vancomycin, was co-polymerized with the cross-linker ethylene dimethacrylate (EDMA) in the presence of methanol and dimethyl sulfoxide as the selected porogens. The co-polymerization conditions were systematically optimized in order to obtain satisfactory column performance. Adequate permeability, stability and column morphology were observed for the optimized poly(ICNEML-vancomycin-co-EDMA) monolith. A series of chiral drugs were evaluated on the monolith in either several other beta-blockers. The proposed single-step approach not only resulted in a vancomycin functionalized organer polar organic-phase or reversed-phase modes. After the optimization of separation conditions, baseline or partial enantioseparation were obtained for series of drugs including thalidomide, colchicine, carteolol, salbutamol, clenbuterol andic polymer-based monolith with acceptable performance, but also significantly simplified the preparation procedure by reducing time and labor. |
| Databáze: | OpenAIRE |
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