| Autor: |
Huiwen Che, Tatjana Jatsenko, Liesbeth Lenaerts, Luc Dehaspe, Leen Vancoillie, Nathalie Brison, Ilse Parijs, Kris Van Den Bogaert, Daniela Fischerova, Ruben Heremans, Chiara Landolfo, Antonia Carla Testa, Adriaan Vanderstichele, Lore Liekens, Valentina Pomella, Agnieszka Wozniak, Christophe Dooms, Els Wauters, Sigrid Hatse, Kevin Punie, Patrick Neven, Hans Wildiers, Sabine Tejpar, Diether Lambrechts, An Coosemans, Dirk Timmerman, Peter Vandenberghe, Frédéric Amant, Joris Robert Vermeesch |
| Přispěvatelé: |
Obstetrics and Gynaecology, CCA - Cancer biology and immunology, ARD - Amsterdam Reproduction and Development |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Clinical chemistry, 68(9), 1164-1176. American Association for Clinical Chemistry Inc. |
| ISSN: |
0009-9147 |
| Popis: |
Background Cell-free DNA (cfDNA) analysis holds great promise for non-invasive cancer screening, diagnosis, and monitoring. We hypothesized that mining the patterns of cfDNA shallow whole-genome sequencing datasets from patients with cancer could improve cancer detection. Methods By applying unsupervised clustering and supervised machine learning on large cfDNA shallow whole-genome sequencing datasets from healthy individuals (n = 367) and patients with different hematological (n = 238) and solid malignancies (n = 320), we identified cfDNA signatures that enabled cancer detection and typing. Results Unsupervised clustering revealed cancer type-specific sub-grouping. Classification using a supervised machine learning model yielded accuracies of 96% and 65% in discriminating hematological and solid malignancies from healthy controls, respectively. The accuracy of disease type prediction was 85% and 70% for the hematological and solid cancers, respectively. The potential utility of managing a specific cancer was demonstrated by classifying benign from invasive and borderline adnexal masses with an area under the curve of 0.87 and 0.74, respectively. Conclusions This approach provides a generic analytical strategy for non-invasive pan-cancer detection and cancer type prediction. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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