Primary Step Towards In Situ Detection of Chemical Biomarkers in the UNIVERSE via Liquid-Based Analytical System: Development of an Automated Online Trapping/Liquid Chromatography System
| Autor: | Claude Geffroy-Rodier, Pauline Poinot, Audrey Allavena, Bertrand Leroux, Balkis Eddhif, Marc David, Robert Sternberg, Thomas Ribette |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
In situ
Materials science Pharmaceutical Science Trapping Mass spectrometry 01 natural sciences Article Analytical Chemistry Trap (computing) lcsh:QD241-441 lcsh:Organic chemistry Tandem Mass Spectrometry 0103 physical sciences Drug Discovery Humans liquid chromatography Sensitivity (control systems) Physical and Theoretical Chemistry Amino Acids Organic Chemicals 010303 astronomy & astrophysics Detection limit Chromatography 010401 analytical chemistry Organic Chemistry Ranging 0104 chemical sciences space instrumentation Chemistry (miscellaneous) trapping system Molecular Medicine Polar Solar System oligopeptides Biomarkers Chromatography Liquid |
| Zdroj: | Molecules, Vol 24, Iss 7, p 1429 (2019) Molecules Volume 24 Issue 7 |
| ISSN: | 1420-3049 |
| Popis: | The search for biomarkers in our solar system is a fundamental challenge for the space research community. It encompasses major difficulties linked to their very low concentration levels, their ambiguous origins (biotic or abiotic), as well as their diversity and complexity. Even if, in 40 years&rsquo time, great improvements in sample pre-treatment, chromatographic separation and mass spectrometry detection have been achieved, there is still a need for new in situ scientific instrumentation. This work presents an original liquid chromatographic system with a trapping unit dedicated to the one-pot detection of a large set of non-volatile extra-terrestrial compounds. It is composed of two units, monitored by a single pump. The first unit is an online trapping unit able to trap polar, apolar, monomeric and polymeric organics. The second unit is an online analytical unit with a high-resolution Q-Orbitrap mass spectrometer. The designed single pump system was as efficient as a laboratory dual-trap LC system for the analysis of amino acids, nucleobases and oligopeptides. The overall setup significantly improves sensitivity, providing limits of detection ranging from ppb to ppt levels, thus meeting with in situ enquiries. |
| Databáze: | OpenAIRE |
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