T lymphocyte recognition sites on peripheral nerve myelin P0 protein
| Autor: | K.V. Toyka, H.-P. Hartung, H. Grosse-Wilde, Christopher Linington, M. Pette, C. Gengaroli |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male T-Lymphocytes T cell Molecular Sequence Data Immunology Peptide Biology Lymphocyte Activation Cell Line Myelin Antigen Homologous chromosome medicine Extracellular Animals Humans Immunology and Allergy Amino Acid Sequence Peripheral Nerves chemistry.chemical_classification T lymphocyte Peptide Fragments Rats medicine.anatomical_structure Neurology chemistry Cytoplasm Cattle Female Neurology (clinical) Myelin P0 Protein Myelin Proteins |
| Zdroj: | Journal of Neuroimmunology. 54:29-34 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/0165-5728(94)90227-5 |
| Popis: | Synthetic peptides corresponding to the extracellular and cytoplasmic domain of bovine (b) or rat (r) peripheral myelin P 0 protein were used to establish a total of 50 short-term T cell lines (TCL) from blood of eight healthy subjects. Despite expressing different HLA-DR and HLA-DQ specificities, one or more TCL (range 1–16) specific for peptide bovine P 0 19–38 could be isolated from the blood of each donor. Therefore, this peptide covers an immunodominant T cell recognition site in humans. However, when testing seven bP 0 -19-38-specific TCL derived from blood of two healthy subjects for recognition of the corresponding human P 0 sequence, no TCL showed any proliferative response. Bovine P 0 -19-38 differs in only two amino acid residues from the human peptide. This observation stresses the necessity for using homologous antigens when screening for T cell-mediated autoreactivity to myelin antigens in humans. Unexpectedly, we failed to establish a single P 0 peptide-specific TCL from blood of four patients with acute Guillain-Barre syndrome (GBS), in which P 0 is considered a putative target autoantigen. As already suggested by others, this could indicate that T cell responses to P 0 do not play a pathogenic role in all GBS cases. Alternatively, in these four patients neuritogenic P 0 -specific T lymphocytes may have been sequestrated to peripheral nerves. |
| Databáze: | OpenAIRE |
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