Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas

Autor: Abdellah Benabdelmouna, Audrey Barranger, Béatrice Adeline, Farida Akcha, Thierry Burgeot, Julien Rouxel, Clothilde Heude-Berthelin
Přispěvatelé: Laboratoire d'Écotoxicologie (LEX), Biogéochimie et Ecotoxicologie (BE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Normandie Université (NU), Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Santé, Génétique et Microbiologie des Mollusques (SGMM), Laboratoire d'Écotoxicologie, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Santé, Génétique et Microbiologie des Mollusques (IFREMER SG2M), Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique)
Rok vydání: 2016
Předmět:
Zdroj: Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology
Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology, 2016, 180, pp.23-30. ⟨10.1016/j.cbpc.2015.11.002⟩
Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology, Elsevier, 2016, 180, pp.23-30. ⟨10.1016/j.cbpc.2015.11.002⟩
Comparative Biochemistry And Physiology C-toxicology & Pharmacology (1532-0456) (Elsevier Science Inc), 2016-02, Vol. 180, P. 23-30
ISSN: 1532-0456
Popis: Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography coupled with UV and electrochemical detection analysis showed an increase in 8-oxodGuo levels in both male and female gonads after exposure to diuron. Immunohistochemistry analysis showed the presence of 8-oxodGuo at all stages of male germ cells, from early to mature stages. Conversely, the oxidized base was only present in early germ cell stages in female gonads. These results indicate that male and female genitors underwent oxidative stress following exposure to diuron, resulting in DNA oxidation in both early germ cells and gametes, such as spermatozoa, which could explain the transmission of diuron-induced DNA damage to offspring. Furthermore, immunostaining of early germ cells seems indicates that damages caused by exposure to diuron on germ line not only affect the current sexual cycle but also could affect future gametogenesis.
Databáze: OpenAIRE
Externí odkaz: