Simian Immunodeficiency Virus Infection of CD4+CD8+T Cells in a Macaque with an Unusually High Peripheral CD4+CD8+T Lymphocyte Count
| Autor: | Emmanuel Khatissian, Valérie Monceaux, Marie-Christine Cumont, Bruno Hurtrel, Raphaël Ho Tsong Fang, Jérôme Estaquier |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
CD4-Positive T-Lymphocytes
Interleukin 2 viruses Immunology Population CD4-CD8 Ratio Simian Acquired Immunodeficiency Syndrome CD8-Positive T-Lymphocytes Biology Lymphocyte Activation medicine.disease_cause Interleukin 21 Virology medicine Animals Cytotoxic T cell Lymphocyte Count education education.field_of_study CD28 T lymphocyte Simian immunodeficiency virus Flow Cytometry Macaca mulatta Infectious Diseases DNA Viral Simian Immunodeficiency Virus CD8 medicine.drug |
| Zdroj: | AIDS Research and Human Retroviruses. 19:267-274 |
| ISSN: | 1931-8405 0889-2229 |
| Popis: | We assessed the possible role in vivo CD4(+) CD8(+) T cells as a viral reservoir for simian immunodeficiency virus (SIV), in a macaque with 50% CD4(+) CD8(+) T cells in peripheral blood. During primary infection (day 14) of this rhesus macaque with the pathogenic SIVmac251 strain, proviruses were detected at similar frequencies in CD4(+) CD8(+) T cells (1/10) and CD4(+) T cells (1/10) and at a lower frequency in CD8(+) T cells (1/800). On day 235, no viral DNA was detected in CD8(+) cells, despite the persistent high viral load, indicating that CD8(+) cells do not constitute a reservoir during the chronic phase of SIV infection. Infection induced early lymphopenia of CD4(+), CD4(+) CD8(+), and CD8(+) cells; only the CD8(+) cell population returned to initial levels and expanded further. We found that CD4(+) CD8(+) T cells expressed the costimulatory CD28 molecule less and were more prone to die in vitro after phytohemagglutinin/interleukin 2 stimulation than were CD4(+) T cells. Taken together, massive death of CD4(+) CD8(+) T cells during acute stages of SIV infection may explain why CD8(+) T cells did not represent a major reservoir for SIV at the onset of infection. |
| Databáze: | OpenAIRE |
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