Substitution of the native srfA promoter by constitutive P in two B. subtilis strains and evaluation of the effect on Surfactin production
| Autor: | Christoph Syldatk, Judit Willenbacher, Rudolf Hausmann, Marius Henkel, Teresa Mohr, Thorsten Mascher, Susanne Gebhard |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Operon Bioengineering Bacillus subtilis Peptides Cyclic Applied Microbiology and Biotechnology Microbiology law.invention Lipopeptides 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins law Cell density Peptide Synthases Promoter Regions Genetic Strain (chemistry) biology Quorum Sensing Gene Expression Regulation Bacterial General Medicine biology.organism_classification Surfactin synthetase Recombinant Proteins Quorum sensing 030104 developmental biology chemistry Biochemistry Genes Bacterial Recombinant DNA lipids (amino acids peptides and proteins) Surfactin Biotechnology |
| Zdroj: | Willenbacher, J, Mohr, T, Henkel, M, Gebhard, S, Mascher, T, Syldatk, C & Hausmann, R 2016, ' Substitution of the native srfA promoter by constitutive P veg in two B. subtilis strains and evaluation of the effect on surfactin production ', Journal of Biotechnology, vol. 224, pp. 14-17 . https://doi.org/10.1016/j.jbiotec.2016.03.002 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2016.03.002 |
| Popis: | The genetic enhancement of Surfactin production increasingly gained attention in the last years, since relatively low product yields limit the industrial application of this biosurfactant. The natural quorum sensing regulation of the srfA operon (coding for the Surfactin synthetase) can reasonably be assumed to be the bottleneck of Surfactin synthesis. Therefore, the replacement of the naturally quorum sensing regulated, and herewith cell density dependent, promoter PsrfA against the Bacillus subtilis endogenous and constitutive promoter Pveg was hypothesized to generally enhance Surfactin yields. The markerless promoter replacement was conducted in the two B. subtilis Surfactin producer strains 3A38 and DSM 10T. The promoter substitution led to an enhancement of Surfactin concentrations in the producer strain 3A38, initially producing only minor amounts of Surfactin (0.07 g/L increased to 0.26 g/L). In contrast, promoter exchange in B. subtilis DSM 10T (wild-type strain producing 0.62 g/L Surfactin) did not achieve an enhancement of Surfactin concentrations (detrimental reduction to 0.04 g/L). These findings implicate that Surfactin synthesis is differently regulated in minor and strong Surfactin producer strains. The hypothesized general enhancement of Surfactin yields after substitution of the native promoter was therefore not confirmed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect