Determination of onset of apoptosis in granulosa cells of the preovulatory follicles in the bonnet monkey (Macaca radiata): correlation with mitogen-activated protein kinase activities
| Autor: | J Uma, Rudraiah Medhamurthy, P Muraly, Shalu Verma-Kumar |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
endocrine system medicine.drug_class Ovary DNA laddering Bcl-2-associated X protein Ovarian Follicle Internal medicine Proto-Oncogene Proteins Follicular phase medicine Animals Mitogen-Activated Protein Kinase 9 Mitogen-Activated Protein Kinase 8 Progesterone bcl-2-Associated X Protein Granulosa Cells biology Estradiol Kinase Caspase 3 Follicular atresia Caspase 2 Cell Biology General Medicine Molecular Reproduction Development & Genetics (formed by the merger of DBGL and CRBME) Follicular fluid Endocrinology medicine.anatomical_structure Macaca radiata Reproductive Medicine Follicular Phase Gene Expression Regulation Proto-Oncogene Proteins c-bcl-2 Caspases biology.protein Female Gonadotropin Mitogen-Activated Protein Kinases Gonadotropins Signal Transduction |
| Zdroj: | Biology of reproduction. 69(4) |
| ISSN: | 0006-3363 |
| Popis: | During reproductive life, only a selected few ovarian follicles mature and ovulate, while the vast majority of follicles undergo a degenerative process called atresia. Recent studies have indicated that follicular atresia is mediated through apoptosis of follicular granulosa cells. The objectives of the present study were to determine the time of onset of apoptosis in granulosa cells of preovulatory follicles and to evaluate the consequences of gonadotropin withdrawal on mitogen-activated protein (MAP) kinase activities. Bonnet monkeys (Macaca radiata) undergoing controlled ovarian stimulation cycles were utilized for stimulation of multiple follicles, and granulosa cells were retrieved from preovulatory follicles at 24, 48, 72, and 96 h after stopping gonadotropin treatment. Serum and follicular fluid estradiol concentrations were highest at 24 h but declined precipitously (P < 0.05) to reach the lowest concentrations at 96 h; however, progesterone concentrations during this period did not increase, indicating the absence of luteinization. Quantitative analysis of genomic DNA by 3'-end labeling revealed the presence of low-molecular-weight fragments from 48 h onward, but by agarose gel electrophoresis, DNA laddering could be visualized only after 72 h. Messenger RNA expression for Bax, caspase-2, and caspase-3 increased with the onset of apoptosis. Immunoblot analysis of MAP kinases in lysates of granulosa cells (48-72 h) indicated increased (P < 0.05) levels of phosphorylated extracellular response kinase-1 and -2, Jun N-terminal kinase (JNK)-1 and -2, and p38. However, in vitro kinase assay data indicated that only phospho-JNK and -p38 activities were higher at 72 h compared to 24 h. These results demonstrate that granulosa cells of preovulatory follicles undergo apoptosis and that increased activities of phospho-JNK and -p38 are correlated with apoptosis in the primate. |
| Databáze: | OpenAIRE |
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