Two lamprey Hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome Hedgehogs
| Autor: | Sylvie Rétaux, Shungo Kano, Didier Casane, Joana Osório, Jin-Hua Xiao, Marc Ekker, Ghislaine Magdelenat, Ferenc Müller, Yavor Hadzhiev |
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| Přispěvatelé: | Institut de Neurobiologie Alfred Fessard (INAF), Centre National de la Recherche Scientifique (CNRS), Neurobiologie et Développement (N&eD), Department of Biology, Center for Advanced Research in Environmental Genomics, University of Ottawa [Ottawa], Department of Medical and Molecular Genetics, School of Clinical and Experimental Medicine, University of Birmingham [Birmingham], Laboratoire Evolution, Génomes et Spéciation (LEGS), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Computational Biology/Comparative Sequence Analysis
MESH: Base Sequence MESH: Lampreys Evolutionary Biology/Developmental Evolution Polymerase Chain Reaction 0302 clinical medicine Lampetra MESH: Animals Evolutionary Biology/Genomics MESH: Phylogeny Zebrafish In Situ Hybridization Phylogeny Genetics 0303 health sciences Multidisciplinary biology Lampreys Enhancer Elements Genetic Regulatory sequence Medicine [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Research Article MESH: DNA Primers DNA Complementary Science Sequence alignment MESH: Sequence Homology Nucleic Acid 03 medical and health sciences MESH: In Situ Hybridization Sequence Homology Nucleic Acid Animals Hedgehog Proteins 14. Life underwater Enhancer Hedgehog Gene 030304 developmental biology DNA Primers Evolutionary Biology Base Sequence Lamprey MESH: Polymerase Chain Reaction MESH: DNA Complementary MESH: Hedgehog Proteins biology.organism_classification Developmental Biology/Neurodevelopment MESH: Enhancer Elements Genetic human activities 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2010, 5 (10), pp.e13332. ⟨10.1371/journal.pone.0013332⟩ PLoS ONE, Vol 5, Iss 10, p e13332 (2010) PLoS ONE, 2010, 5 (10), pp.e13332. ⟨10.1371/journal.pone.0013332⟩ |
| ISSN: | 1932-6203 |
| Popis: | International audience; Hedgehog (Hh) genes play major roles in animal development and studies of their evolution, expression and function point to major differences among chordates. Here we focused on Hh genes in lampreys in order to characterize the evolution of Hh signalling at the emergence of vertebrates. Screening of a cosmid library of the river lamprey Lampetra fluviatilis and searching the preliminary genome assembly of the sea lamprey Petromyzon marinus indicate that lampreys have two Hh genes, named Hha and Hhb. Phylogenetic analyses suggest that Hha and Hhb are lamprey-specific paralogs closely related to Sonic/Indian Hh genes. Expression analysis indicates that Hha and Hhb are expressed in a Sonic Hh-like pattern. The two transcripts are expressed in largely overlapping but not identical domains in the lamprey embryonic brain, including a newly-described expression domain in the nasohypophyseal placode. Global alignments of genomic sequences and local alignment with known gnathostome regulatory motifs show that lamprey Hhs share conserved non-coding elements (CNE) with gnathostome Hhs albeit with sequences that have significantly diverged and dispersed. Functional assays using zebrafish embryos demonstrate gnathostome-like midline enhancer activity for CNEs contained in intron2. We conclude that lamprey Hh genes are gnathostome Shh-like in terms of expression and regulation. In addition, they show some lamprey-specific features, including duplication and structural (but not functional) changes in the intronic/regulatory sequences. |
| Databáze: | OpenAIRE |
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