Ameliorative role of trans-ferulic acid on induced oxidative toxicity of rooster semen by β-cyfluthrin during low temperature liquid storage
| Autor: | Abolfazl Ghaniei, Elham Zadeh-Hashem, Morteza Shayan-Nasr, Mohsen Eslami |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Coumaric Acids Rooster Semen Cyfluthrin SF1-1100 Ferulic acid Superoxide dismutase chemistry.chemical_compound Nitriles Pyrethrins Animals Food science trans-ferulic acid cyfluthrin Cryopreservation biology rooster PHYSIOLOGY AND REPRODUCTION Temperature semen General Medicine biology.organism_classification Malondialdehyde Spermatozoa Animal culture Semen Analysis Oxidative Stress chemistry Toxicity biology.protein Sperm Motility Animal Science and Zoology Chickens Toxicant Semen Preservation |
| Zdroj: | Poultry Science Poultry Science, Vol 100, Iss 9, Pp 101308-(2021) |
| ISSN: | 1525-3171 |
| Popis: | Current study was designed to evaluate the effects of β-cyfluthrin, as a toxicant substance, and trans-ferulic acid (trans-FA), as a protective agent, on different parameters of rooster semen upon liquid storage. For this purpose, semen samples of roosters (Ross 308, n = 10, 32-wk-old) were collected twice a week. Good quality samples (≥70% progressive motion) were diluted, pooled and then divided for the purposes of the study. In the first experiment, motility of spermatozoa was evaluated following exposure to different concentrations of β-cyfluthrin (1, 2.5, 5, 10, 20, 40, and 80 µM) at 0, 24, and 48 h of storage. In the second experiment, constant doses of β-cyfluthrin (10 µM) alone or in combination with trans-FA (10, 25 mM) were assessed on motility and viability of spermatozoa at 0, 24, and 48 h time points. Moreover, amounts of malondialdehyde (MDA), total antioxidant capacity (TAC), total nitrate-nitrite, total hydroperoxide (HPO), and activity of superoxide dismutase (SOD) were evaluated in the homogenate of spermatozoa-diluent at studied time points. Results of the first experiment showed that amounts of β-cyfluthrin greater than 5 µM, significantly reduced the motility of spermatozoa at 24 and 48 h of storage (P < 0.05). The second experiment demonstrated that, trans-FA especially at 10, 25 mM doses restored the motility and viability of spermatozoa compared to β-cyfluthrin treated group (P < 0.05). Amounts of MDA (10, 25 mM), hydroperoxide (10, 25, and 50 mM), and nitrate-nitrite (10, 25, and 50 mM) were lower and TAC (10 and 25 mM) were greater in trans-FA + β-cyfluthrin treated groups compared to β-cyfluthrin alone treated samples (P < 0.05). However, activity of SOD did not show significant changes by the treatment (P > 0.05). It seems that trans-FA could ameliorate toxic effect of β-cyfluthrin via reduction of peroxidative (as evident by measurement of MDA) and nitrosative (as evident by measurement of nitrate-nitrite) reactions over cold preservation of rooster semen. |
| Databáze: | OpenAIRE |
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