In vitro anti-biofilm and anti-bacterial activity of Junceella juncea for its biomedical application
| Autor: | Ponnuchamy Kumar, M. Govindaraju, S. Senthamil Selvi |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Conservation of Natural Resources
Colony Count Microbial Microbial Sensitivity Tests In Vitro Techniques Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Bacterial Adhesion Microbiology Spectroscopy Fourier Transform Infrared Botany Escherichia coli Animals Adhesins Bacterial Vibrio cholerae Methanol Biofilm food and beverages Congo Red Salmonella typhi biochemical phenomena metabolism and nutrition Anthozoa In vitro Anti-Bacterial Agents Junceella juncea Biofilms Original Article Anti bacterial Anti biofilm |
| Zdroj: | Asian Pacific Journal of Tropical Biomedicine. 2:930-935 |
| ISSN: | 2221-1691 |
| DOI: | 10.1016/s2221-1691(13)60002-7 |
| Popis: | To investigate the anti-biofilm and anti-bacterial activity of Junceella juncea (J. juncea) against biofilm forming pathogenic strains.Gorgonians were extracted with methanol and analysed with fourier transform infrared spectroscopy. Biofilm forming pathogens were identified by Congo red agar supplemented with sucrose. A quantitative spectrophotometric method was used to monitor in vitro biofilm reduction by microtitre plate assay. Anti-bacterial activity of methanolic gorgonian extract (MGE) was carried out by disc diffusion method followed by calculating the percentage of increase with crude methanol (CM).The presence of active functional group was exemplified by FT-IR spectroscopy. Dry, black, crystalline colonies confirm the production of extracellular polymeric substances responsible for biofilm formation in Congo red agar. MGE exhibited potential anti-biofilm activity against all tested bacterial strains. The anti-bacterial activity of methanolic extract was comparably higher in Salmonella typhii followed by Escherichia coli, Vibrio cholerae and Shigella flexneri. The overall percentage of increase was higher by 50.2% to CM.To conclude, anti-biofilm and anti-bacterial efficacy of J. juncea is impressive over biofilm producing pathogens and are good source for novel anti-bacterial compounds. |
| Databáze: | OpenAIRE |
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