Increased sensitivity of the card agglutination test CATT/Trypanosoma brucei gambiense by inhibition of complement
Autor: | Eddy Magnus, L Verhelst, N. Van Meirvenne, R Pansaerts |
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Rok vydání: | 1998 |
Předmět: |
Serial dilution
Trypanosoma brucei gambiense Veterinary (miscellaneous) Antibodies Protozoan Antigens Protozoan Trypanosoma brucei Agglutination Tests parasitic diseases medicine Animals Humans Mass Screening Complement Activation Mass screening biology Kinetoplastida biology.organism_classification medicine.disease Virology Card agglutination Complement system Africa Western Trypanosomiasis African Infectious Diseases Insect Science Trypanosoma Parasitology Rabbits Trypanosomiasis |
Zdroj: | Acta Tropica. 70:349-354 |
ISSN: | 0001-706X |
DOI: | 10.1016/s0001-706x(98)00046-1 |
Popis: | CATT/Trypanosoma brucei (T.b.) gambiense is an antibody detection test currently used in field surveys on Gambian sleeping sickness. The screening test is usually performed on a drop of freshly collected heparinized blood, followed by a more specific confirmation test on diluted blood, plasma or serum. This approach may be biased by the occurrence of a complement-mediated prozone phenomenon causing lower test sensitivity at lower sample dilutions. A simple remedy is by addition of a Ca2+ chelating agent such as EDTA. |
Databáze: | OpenAIRE |
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