Infection and Immunity Mediated by the Carbohydrate Recognition Domain of theEntamoeba histolyticaGal/GalNAc Lectin
| Autor: | William A. Petri, Aleida Eubanks, David M. Lyerly, Lauren A. Lockhart, James M. Dodson, Jason Napodano, Paul W. Lenkowski, Terry F. G. H. Jackson, Barbara J. Mann |
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| Rok vydání: | 1999 |
| Předmět: |
Carbohydrates
Protozoan Proteins Antigens Protozoan Ligands Epitope Microbiology Entamoeba histolytica Immune system Antigen Lectins Carbohydrate Conformation medicine Animals Humans Immunology and Allergy Binding Sites Membrane Glycoproteins Entamoebiasis biology Hepatocyte Growth Factor Lectin biology.organism_classification Virology eye diseases Infectious Diseases Expression cloning biology.protein Calcium Hepatocyte growth factor Antibody medicine.drug |
| Zdroj: | The Journal of Infectious Diseases. 179:460-466 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/314610 |
| Popis: | Entamoeba histolytica causes invasive amebiasis, a major parasitic disease of the developing world, whose primary symptoms are liver abscess and colitis. All strains of E. histolytica express a 260-kDa surface Gal/GalNAc lectin that is antigenically conserved and immunogenic. The lectin is required for adherence to human intestinal epithelial cells and contact-dependent killing of immune effector cells. By expression cloning, the carbohydrate recognition domain (CRD) was identified within the lectin heavy-subunit cysteine-rich region. Of interest for a hepatic parasite, the CRD had sequence identity to the receptor-binding domain of hepatocyte growth factor (HGF) and competed with HGF for binding to the c-Met HGF receptor. In an animal model of invasive disease, immunization with the CRD inhibited liver-abscess formation, yet in humans, a naturally acquired immune response against the CRD did not persist. |
| Databáze: | OpenAIRE |
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