The effect of interferon gamma on conventional fractionated radiation-induced damage and fibrosis in the pelvic tissue of rabbits
| Autor: | Yan Lan Chai, Jin Su, Jiquan Wang, Shao Min Che, Fan Shi, Zi Liu, Yunyi Yang, Juan Wang |
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| Rok vydání: | 2016 |
| Předmět: |
Pathology
medicine.medical_specialty pelvic irradiation damage Therapeutics and Clinical Risk Management medicine.medical_treatment 0206 medical engineering rabbit 02 engineering and technology 03 medical and health sciences 0302 clinical medicine Ureter Downregulation and upregulation Fibrosis medicine Pharmacology (medical) Interferon gamma General Pharmacology Toxicology and Pharmaceutics IFN-γ Saline Fractionated radiation Original Research 030203 arthritis & rheumatology Chemical Health and Safety biology business.industry Collagen iii General Medicine Transforming growth factor beta medicine.disease 020601 biomedical engineering TGF-β1 mRNA medicine.anatomical_structure biology.protein collagen III business Safety Research medicine.drug |
| Zdroj: | Therapeutics and Clinical Risk Management |
| ISSN: | 1178-203X |
| DOI: | 10.2147/tcrm.s101346 |
| Popis: | Yunyi Yang, Zi Liu, Juan Wang, Yanlan Chai, Jin Su, Fan Shi, Jiquan Wang, Shao Min Che Department of Radiation Oncology, The First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an, Shanxi, People’s Republic of China Abstract: We aim to investigate the effect of interferon gamma (IFN-γ) on conventional fractionated radiation–induced damage and fibrosis in ureter and colorectal mucosa. Fifty-two rabbits were randomly divided into three groups comprising a conventional radiation group, an IFN-γ group, and a control group. X-rays were used to irradiate the pelvic tissues of the rabbits in the IFN-γ and conventional radiation groups. Five days after radiation exposure, the rabbits in the IFN-γ group were administered 250,000U/kg IFN-γ intramuscularly once a week for 5weeks. The rabbits in the conventional radiation group received 5.0mL/kg saline. The rabbits were sacrificed at 4, 8, 12, and 16weeks postradiation, and the rectal and ureteral tissues within the radiation areas were collected. The results showed that the morphology of rectal and ureteral tissues was changed by X-ray radiation. The degree of damage at 4, 8, and 12weeks, but not at 16weeks, postradiation was significantly different between the IFN-γ and conventional radiation groups. The expression of transforming growth factor beta 1 mRNA in the ureter and colorectal mucosa of the IFN-γ group was significantly lower than that in the conventional radiation group at 4, 8, 12, and 16weeks postradiation, but it was still higher than that in the control group. There were significant differences in the expression of collagen III among the three groups. IFN-γ can inhibit the radiation-induced upregulation of transforming growth factor beta 1 mRNA and collagen III protein in the ureter and colorectal mucosa and attenuate radiation-induced damage and fibrosis. Keywords: IFN-γ, pelvic irradiation damage, TGF-β1 mRNA, collagen III, rabbit |
| Databáze: | OpenAIRE |
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